Table 2 Purification of the PhXyl
Purification steps | Total activitya (IU) | Total protein (mg) | Specific activity (IU/mg) | Recovery (%) | Fold purification |
|---|---|---|---|---|---|
Culture filtrate | 1155 | 67.14 | 17.21 | 100.00 | 1 |
Ammonium sulphate precipitation | 1099 | 17.86 | 61.42 | 95.20 | 3.56 |
QAE Sephadex A50 Chromatography | 752 | 7.66 | 98.17 | 68.42 | 5.70 |
Gel filtration Chromatography (Sephacryl-200) | 399.46 | 2.79 | 143.17 | 53.12 | 8.3 |