Skip to main content

Table 2 Purification of the PhXyl

From: Purification and characterization of an extracellular β-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast

Purification steps Total activitya (IU) Total protein (mg) Specific activity (IU/mg) Recovery (%) Fold purification
Culture filtrate 1155 67.14 17.21 100.00 1
Ammonium sulphate precipitation 1099 17.86 61.42 95.20 3.56
QAE Sephadex A50 Chromatography 752 7.66 98.17 68.42 5.70
Gel filtration Chromatography (Sephacryl-200) 399.46 2.79 143.17 53.12 8.3
  1. The values show the average of three independent experiments
  2. aβ-xylosidase activity was assayed using pNPX