Table 1 Purification of cellulase Spr Cel8A of Serratia proteamaculans
Step | Protein (mg) | Total activity (U) | Specific-activity (U/mg) | Purification fold | Recovery yield (%) |
|---|---|---|---|---|---|
Soluble fraction of cell lysis | 43.5 | 8.0 | 0.18 | 1 | 100 |
Ni–NTA affinity chromatography | 1 | 0.85 | 0.85 | 4.72 | 10.58 |