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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: Expression, purification and characterization of an endoglucanase from Serratia proteamaculans CDBB-1961, isolated from the gut of Dendroctonus adjunctus (Coleoptera: Scolytinae)

Fig. 3

Effect of pH and temperature on Spr Cel8A activity and stability. a pH effect with different buffers; citrate phosphate (pH 3–7), phosphates (pH 6-8) and glycine-NaOH (8.5–10.5) and incubated at 40 ºC for 30 min. b pH stability, protein was incubated in different buffer, mentioned above, at different pH values, ranging from 3.0 to 10.5, at 28 ºC for 1 h. c Temperature effect, the enzyme was incubated with 0.5 % (w/v) CMC dissolved in 50 mM citrate phosphate buffer, pH 7.0, for 30 min at different temperatures (15–55 ºC). All tests were performed in triplicate and error bars indicate standard deviations

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