Development of a novel strategy for fungal transformation based on a mutant locus conferring carboxin-resistance in Magnaporthe oryzae

AMB Express

Table 1 Phenotypic summary of pMoC-eGFP targeted integration mutants

Strains	Conidiation (×10⁵ spores/cm²)	Conidium size (um)		Germination rate (%)	Appressorium formation (%)	Colony diameter (mm)
Strains	Conidiation (×10⁵ spores/cm²)	Length	Width	Germination rate (%)	Appressorium formation (%)	CM	MM	OM	RDC	V8
Guy11	1.03 ± 0.16^a	23.14 ± 1.91^a	8.83 ± 1.29^a	95.43 ± 1.13^a	98.04 ± 1.96^a	38.23 ± 0.25^a	32.30 ± 0.70^a	33.63 ± 0.57^a	33.93 ± 0.31^a	35.03 ± 0.15^a
M1	1.08 ± 0.08^a	24.43 ± 2.08^a	8.67 ± 1.38^a	95.43 ± 2.99^a	99.35 ± 1.13^a	38.20 ± 0.30^a	31.77 ± 0.25^a	33.77 ± 0.25^a	33.77 ± 0.25^a	34.83 ± 0.15^a
M2	1.03 ± 0.21^a	22.61 ± 1.69^a	8.41 ± 1.15^a	94.12 ± 1.96^a	97.39 ± 1.13^a	37.83 ± 0.29^a	31.93 ± 0.12^a	33.83 ± 0.25^a	33.63 ± 0.45^a	34.70 ± 0.26^a
M3	0.94 ± 0.08^a	23.10 ± 1.79^a	8.99 ± 1.25^a	93.47 ± 4.08^a	98.69 ± 1.13^a	38.00 ± 0.20^a	32.10 ± 0.36^a	33.90 ± 0.36^a	34.07 ± 0.40^a	34.83 ± 0.15^a
M4	1.03 ± 0.08^a	23.10 ± 1.79^a	8.88 ± 1.09^a	94.12 ± 1.96^a	97.39 ± 2. 39^a	37.77 ± 0.25^a	31.53 ± 0.50^a	33.43 ± 0.21^a	33.83 ± 0.35^a	34.80 ± 0.20^a
M5	0.98 ± 0.14^a	21.79 ± 2.32^a	8.66 ± 1.04^a	94.77 ± 1.13^a	98.69 ± 1.13^a	37.90 ± 0.36^a	32.00 ± 0.10^a	33.77 ± 0.25^a	33.83 ± 0.15^a	34.77 ± 0.38^a

Conidiation were measured by counting the number of conidia that harvested with 5 ml of sterilized water from 10-day-old RDC agar plates. The conidia sizes were measured with width and length from 99 conidia of each strain. Fungal mycelial growth rate was statistically analyzed by measuring the colony diameter of each strain on five different artificial media after 5 days after inoculation. For conidium germination and appressorium formation, drops (20 μL) of conidial suspension (5 × 10⁴ spores/mL) were inoculated onto a hydrophobic coverslip and placed under humid conditions at 28 °C. Conidia germination and appressoria formation were observed by microscopic examination of at least 99 conidia per replicate at each time point. All experiments were repeated three times, and representative results from one experiment are shown
* Lowercase letters indicate non-significant difference estimated by Duncan’s test (P < 0.05)