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Table 1 Phenotypic summary of pMoC-eGFP targeted integration mutants

From: Development of a novel strategy for fungal transformation based on a mutant locus conferring carboxin-resistance in Magnaporthe oryzae

Strains Conidiation (×105 spores/cm2) Conidium size (um) Germination rate (%) Appressorium formation (%) Colony diameter (mm)
Length Width CM MM OM RDC V8
Guy11 1.03 ± 0.16a 23.14 ± 1.91a 8.83 ± 1.29a 95.43 ± 1.13a 98.04 ± 1.96a 38.23 ± 0.25a 32.30 ± 0.70a 33.63 ± 0.57a 33.93 ± 0.31a 35.03 ± 0.15a
M1 1.08 ± 0.08a 24.43 ± 2.08a 8.67 ± 1.38a 95.43 ± 2.99a 99.35 ± 1.13a 38.20 ± 0.30a 31.77 ± 0.25a 33.77 ± 0.25a 33.77 ± 0.25a 34.83 ± 0.15a
M2 1.03 ± 0.21a 22.61 ± 1.69a 8.41 ± 1.15a 94.12 ± 1.96a 97.39 ± 1.13a 37.83 ± 0.29a 31.93 ± 0.12a 33.83 ± 0.25a 33.63 ± 0.45a 34.70 ± 0.26a
M3 0.94 ± 0.08a 23.10 ± 1.79a 8.99 ± 1.25a 93.47 ± 4.08a 98.69 ± 1.13a 38.00 ± 0.20a 32.10 ± 0.36a 33.90 ± 0.36a 34.07 ± 0.40a 34.83 ± 0.15a
M4 1.03 ± 0.08a 23.10 ± 1.79a 8.88 ± 1.09a 94.12 ± 1.96a 97.39 ± 2. 39a 37.77 ± 0.25a 31.53 ± 0.50a 33.43 ± 0.21a 33.83 ± 0.35a 34.80 ± 0.20a
M5 0.98 ± 0.14a 21.79 ± 2.32a 8.66 ± 1.04a 94.77 ± 1.13a 98.69 ± 1.13a 37.90 ± 0.36a 32.00 ± 0.10a 33.77 ± 0.25a 33.83 ± 0.15a 34.77 ± 0.38a
  1. Conidiation were measured by counting the number of conidia that harvested with 5 ml of sterilized water from 10-day-old RDC agar plates. The conidia sizes were measured with width and length from 99 conidia of each strain. Fungal mycelial growth rate was statistically analyzed by measuring the colony diameter of each strain on five different artificial media after 5 days after inoculation. For conidium germination and appressorium formation, drops (20 μL) of conidial suspension (5 × 104 spores/mL) were inoculated onto a hydrophobic coverslip and placed under humid conditions at 28 °C. Conidia germination and appressoria formation were observed by microscopic examination of at least 99 conidia per replicate at each time point. All experiments were repeated three times, and representative results from one experiment are shown
  2. * Lowercase letters indicate non-significant difference estimated by Duncan’s test (P < 0.05)