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Table 1 Strains and plasmids used in this study
| Strains and plasmids | Description | Source |
|---|---|---|
| Strains | ||
| W3110 | Wild type E. coli strain ATCC 27325 | ATCC |
| BL21(DE3) | E. coli strain for protein expression | Novagen |
| JM109 | Host E. coli for gene cloning | Novagen |
| BL21(DE3)/pET20b(+) | BL21(DE3) containing pET20b(+) | This study |
| BL21(DE3)/pET20b-gadB | BL21(DE3) containing pET20b-gadB | This study |
| BL21(DE3)/pET20b-pelB-gadB | BL21(DE3) containing pET20b-pelB-gadB | This study |
| BL21(DE3)/pET20b-torA-gadB | BL21(DE3) containing pET20b-torA-gadB | This study |
| Plasmids | ||
| pET20b(+) | E. coli protein expression vector | Novagen |
| pET20b-gadB | pET20b(+) harboring gadB without signal sequence | This study |
| pET20b-pelB-gadB | pET20b(+) harboring gadB with signal sequence pelB | This study |
| pET20b-torA-gadB | pET20b(+) harboring gadB with signal sequence torA | This study |
