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Table 1 Strains and plasmids used in this study

From: Extracellular expression of glutamate decarboxylase B in Escherichia coli to improve gamma-aminobutyric acid production

Strains and plasmids Description Source
W3110 Wild type E. coli strain ATCC 27325 ATCC
BL21(DE3) E. coli strain for protein expression Novagen
JM109 Host E. coli for gene cloning Novagen
BL21(DE3)/pET20b(+) BL21(DE3) containing pET20b(+) This study
BL21(DE3)/pET20b-gadB BL21(DE3) containing pET20b-gadB This study
BL21(DE3)/pET20b-pelB-gadB BL21(DE3) containing pET20b-pelB-gadB This study
BL21(DE3)/pET20b-torA-gadB BL21(DE3) containing pET20b-torA-gadB This study
pET20b(+) E. coli protein expression vector Novagen
pET20b-gadB pET20b(+) harboring gadB without signal sequence This study
pET20b-pelB-gadB pET20b(+) harboring gadB with signal sequence pelB This study
pET20b-torA-gadB pET20b(+) harboring gadB with signal sequence torA This study