Table 1 Strains and plasmids used in this study
Strains and plasmids | Description | Source |
|---|---|---|
Strains | ||
W3110 | Wild type E. coli strain ATCC 27325 | ATCC |
BL21(DE3) | E. coli strain for protein expression | Novagen |
JM109 | Host E. coli for gene cloning | Novagen |
BL21(DE3)/pET20b(+) | BL21(DE3) containing pET20b(+) | This study |
BL21(DE3)/pET20b-gadB | BL21(DE3) containing pET20b-gadB | This study |
BL21(DE3)/pET20b-pelB-gadB | BL21(DE3) containing pET20b-pelB-gadB | This study |
BL21(DE3)/pET20b-torA-gadB | BL21(DE3) containing pET20b-torA-gadB | This study |
Plasmids | ||
pET20b(+) | E. coli protein expression vector | Novagen |
pET20b-gadB | pET20b(+) harboring gadB without signal sequence | This study |
pET20b-pelB-gadB | pET20b(+) harboring gadB with signal sequence pelB | This study |
pET20b-torA-gadB | pET20b(+) harboring gadB with signal sequence torA | This study |