Strains
|
W3110
|
Wild type E. coli strain ATCC 27325
|
ATCC
|
BL21(DE3)
|
E. coli strain for protein expression
|
Novagen
|
JM109
|
Host E. coli for gene cloning
|
Novagen
|
BL21(DE3)/pET20b(+)
|
BL21(DE3) containing pET20b(+)
|
This study
|
BL21(DE3)/pET20b-gadB
|
BL21(DE3) containing pET20b-gadB
|
This study
|
BL21(DE3)/pET20b-pelB-gadB
|
BL21(DE3) containing pET20b-pelB-gadB
|
This study
|
BL21(DE3)/pET20b-torA-gadB
|
BL21(DE3) containing pET20b-torA-gadB
|
This study
|
Plasmids
|
pET20b(+)
|
E. coli protein expression vector
|
Novagen
|
pET20b-gadB
|
pET20b(+) harboring gadB without signal sequence
|
This study
|
pET20b-pelB-gadB
|
pET20b(+) harboring gadB with signal sequence pelB
|
This study
|
pET20b-torA-gadB
|
pET20b(+) harboring gadB with signal sequence torA
|
This study
|