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Fig. 3 | AMB Express

Fig. 3

From: Extracellular expression of glutamate decarboxylase B in Escherichia coli to improve gamma-aminobutyric acid production

Fig. 3

Optimization of GadB expression and secretion in BL21(DE3)/pET20b-torA-gadB. a Effect of temperature and IPTG on cell density, intracellular and extracellular activities. b Effect of NaCl and Glycine on cell density, intracellular and extracellular activities. Cells were grown in TB medium at 37 °C for 36 h. The error bars indicate the standard deviations from three parallel experiments

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