Fig. 3From: Extracellular expression of glutamate decarboxylase B in Escherichia coli to improve gamma-aminobutyric acid productionOptimization of GadB expression and secretion in BL21(DE3)/pET20b-torA-gadB. a Effect of temperature and IPTG on cell density, intracellular and extracellular activities. b Effect of NaCl and Glycine on cell density, intracellular and extracellular activities. Cells were grown in TB medium at 37 °C for 36 h. The error bars indicate the standard deviations from three parallel experimentsBack to article page