Fig. 2

BL21(DE3)/pET20b(+), BL21(DE3)/pET20b-gadB, BL21(DE3)/pET20b- torA-gadB and BL21(DE3)/pET20b-pelB-gadB cells were grown in TB medium at 37 °C for 36 h. a The intracellular and extracellular fractions from different cells were analyzed by using SDS-PAGE. b Intracellular and extracellular gadB activities and cell densities of different strains were determined. The molecular mass of GadB is about 53 kDa. M marker of standard protein; Intra intracellular sample; Extra extracellular sample; Total the sum GadB activity of intracellular and extracellular samples