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Table 2 Relative densities of hydrolysis spots produced by commercial lipases at different temperatures on P(3HB-co-4HB)

From: Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film

Temp. (°C) Relative densities of hydrolysis spots produced by lipase from different sources
C. antarctica C. rugosa M. javanicus Porcine pancreas P. cepacia P. fluorescens R. arrhizus R. niveus R. oryzae
Buffer 1.00 ± 0.24a 1.00 ± 0.08a 1.00 ± 0.22a 1.00 ± 0.29a 1.00 ± 0.17a 1.00 ± 0.18a 1.00 ± 0.02a 1.00 ± 0.14a 1.00 ± 0.17a
15 10.04 ± 0.33ab 2.42 ± 0.58ab 1.07 ± 0.21a 1.25 ± 0.18a 2.15 ± 0.17abc 4.62 ± 0.10bc 4.69 ± 0.28ab 3.80 ± 0.23b 1.92 ± 0.32a
20 11.73 ± 0.28bc 2.73 ± 0.33abc 1.13 ± 0.50a 1.37 ± 0.41a 2.02 ± 0.14ab 3.07 ± 0.31b 11.10 ± 0.30bc 3.24 ± 0.08b 2.07 ± 0.26a
25 16.18 ± 0.36bc 3.93 ± 0.56abcd 4.41 ± 0.34ab 1.30 ± 0.38a 2.33 ± 0.34abc 3.72 ± 0.21b 8.99 ± 0.18b 4.60 ± 0.23b 3.24 ± 0.48b
30 17.94 ± 0.11bc 3.36 ± 0.33abc 4.52 ± 0.07ab 3.17 ± 0.30ab 5.28 ± 0.25 cd 3.60 ± 0.33b 19.79 ± 0.23d 6.95 ± 0.10c 2.60 ± 0.07b
35 20.66 ± 0.04c 5.32 ± 0.15bcd 10.29 ± 0.52bc 4.16 ± 0.29b 7.72 ± 0.30d 6.60 ± 0.14c 24.54 ± 0.02d 7.20 ± 0.11c 7.25 ± 0.11b
40 17.58 ± 0.18bc 5.61 ± 0.11 cd 8.34 ± 0.69abc 7.89 ± 0.18c 8.14 ± 0.14d 10.62 ± 0.03d 18.26 ± 0.27 cd 12.66 ± 0.04d 8.30 ± 0.14b
45 14.63 ± 0.29bc 6.66 ± 0.08d 15.12 ± 0.06c 8.47 ± 0.24c 5.01 ± 0.17bcd 8.78 ± 0.08d 23.09 ± 0.04d 11.70 ± 0.10d 6.63 ± 0.22b
  1. Buffer indicates PBS pH 7.4 without addition of lipase. The data are mean of triplicate. Mean data accompanied by different superscript alphabets are significantly different (Tukey’s HSD test, p < 0.05). The assay was conducted for 30 min