Skip to main content

Table 2 Relative densities of hydrolysis spots produced by commercial lipases at different temperatures on P(3HB-co-4HB)

From: Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film

Temp. (°C)

Relative densities of hydrolysis spots produced by lipase from different sources

C. antarctica

C. rugosa

M. javanicus

Porcine pancreas

P. cepacia

P. fluorescens

R. arrhizus

R. niveus

R. oryzae

Buffer

1.00 ± 0.24a

1.00 ± 0.08a

1.00 ± 0.22a

1.00 ± 0.29a

1.00 ± 0.17a

1.00 ± 0.18a

1.00 ± 0.02a

1.00 ± 0.14a

1.00 ± 0.17a

15

10.04 ± 0.33ab

2.42 ± 0.58ab

1.07 ± 0.21a

1.25 ± 0.18a

2.15 ± 0.17abc

4.62 ± 0.10bc

4.69 ± 0.28ab

3.80 ± 0.23b

1.92 ± 0.32a

20

11.73 ± 0.28bc

2.73 ± 0.33abc

1.13 ± 0.50a

1.37 ± 0.41a

2.02 ± 0.14ab

3.07 ± 0.31b

11.10 ± 0.30bc

3.24 ± 0.08b

2.07 ± 0.26a

25

16.18 ± 0.36bc

3.93 ± 0.56abcd

4.41 ± 0.34ab

1.30 ± 0.38a

2.33 ± 0.34abc

3.72 ± 0.21b

8.99 ± 0.18b

4.60 ± 0.23b

3.24 ± 0.48b

30

17.94 ± 0.11bc

3.36 ± 0.33abc

4.52 ± 0.07ab

3.17 ± 0.30ab

5.28 ± 0.25 cd

3.60 ± 0.33b

19.79 ± 0.23d

6.95 ± 0.10c

2.60 ± 0.07b

35

20.66 ± 0.04c

5.32 ± 0.15bcd

10.29 ± 0.52bc

4.16 ± 0.29b

7.72 ± 0.30d

6.60 ± 0.14c

24.54 ± 0.02d

7.20 ± 0.11c

7.25 ± 0.11b

40

17.58 ± 0.18bc

5.61 ± 0.11 cd

8.34 ± 0.69abc

7.89 ± 0.18c

8.14 ± 0.14d

10.62 ± 0.03d

18.26 ± 0.27 cd

12.66 ± 0.04d

8.30 ± 0.14b

45

14.63 ± 0.29bc

6.66 ± 0.08d

15.12 ± 0.06c

8.47 ± 0.24c

5.01 ± 0.17bcd

8.78 ± 0.08d

23.09 ± 0.04d

11.70 ± 0.10d

6.63 ± 0.22b

  1. Buffer indicates PBS pH 7.4 without addition of lipase. The data are mean of triplicate. Mean data accompanied by different superscript alphabets are significantly different (Tukey’s HSD test, p < 0.05). The assay was conducted for 30 min