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Table 1 Relative densities of hydrolysis spots produced by commercial lipases at pH 1–11 on P(3HB-co-4HB)

From: Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film

pH Relative densities of hydrolysis spots produced by lipase from different sources
C. antarctica C. rugosa M. javanicus Porcine pancreas P. cepacia P. fluorescens R. arrhizus R. niveus R. oryzae
Buffer 1.00 ± 0.30a 1.00 ± 0.15a 1.00 ± 0.32a 1.00 ± 0.11a 1.00 ± 0.15a 1.00 ± 0.27a 1.00 ± 0.18a 1.00 ± 0.20a 1.00 ± 0.21a
1 0.70 ± 0.87a 0.82 ± 0.24a 1.05 ± 0.30a 0.84 ± 0.77a 0.72 ± 0.49a 1.26 ± 0.79a 5.77 ± 0.42abc 3.90 ± 0.21ab 1.19 ± 0.39a
2 1.35 ± 0.41a 0.89 ± 0.26a 1.03 ± 0.20a 0.55 ± 0.50a 0.88 ± 1.28a 1.09 ± 0.45a 5.53 ± 0.57abc 2.03 ± 0.32a 1.15 ± 0.14a
3 25.98 ± 0.30d 0.80 ± 0.11a 1.10 ± 0.55a 1.04 ± 0.47a 2.53 ± 0.15a 1.28 ± 0.91a 6.03 ± 0.53abc 6.41 ± 0.54abc 1.19 ± 0.29a
4 14.27 ± 0.20bc 4.24 ± 0.26b 0.83 ± 0.28a 0.91 ± 0.65a 15.51 ± 0.40abcd 2.29 ± 0.30ab 27.65 ± 0.49bcd 8.24 ± 0.23bcd 1.20 ± 0.20a
5 20.93 ± 0.25 cd 6.11 ± 0.09c 3.56 ± 0.42ab 0.81 ± 0.53a 19.05 ± 0.10bcd 4.36 ± 0.34ab 26.77 ± 0.59bcd 11.51 ± 0.36cde 1.20 ± 0.16a
6 30.66 ± 0.30d 7.26 ± 0.05c 3.78 ± 0.39ab 5.30 ± 0.24bc 17.47 ± 0.25abcd 4.48 ± 0.28ab 29.37 ± 0.04 cd 13.35 ± 0.13de 5.19 ± 0.60b
7 51.64 ± 0.01e 6.77 ± 0.09c 8.35 ± 0.41b 8.91 ± 0.36c 20.85 ± 0.20 cd 7.45 ± 0.48abc 23.60 ± 0.77abcd 14.79 ± 0.26e 3.77 ± 0.40ab
8 6.40 ± 0.39ab 0.83 ± 0.33a 6.53 ± 0.69b 8.58 ± 0.39c 11.45 ± 0.31abc 17.59 ± 0.30c 35.18 ± 0.22d 17.34 ± 0.03e 5.20 ± 0.15b
9 1.20 ± 0.23a 0.71 ± 0.22a 0.91 ± 0.29a 3.86 ± 0.22ab 10.15 ± 0.26abc 17.26 ± 0.25c 24.28 ± 0.20abcd 1.38 ± 0.42a 6.69 ± 0.08b
10 0.99 ± 0.27a 0.80 ± 0.13a 0.88 ± 0.55a 1.32 ± 0.41a 8.27 ± 0.02abc 18.26 ± 0.41c 3.15 ± 0.31ab 1.27 ± 0.25a 1.17 ± 0.35a
11 0.86 ± 0.38a 0.66 ± 0.30a 1.10 ± 0.62a 0.49 ± 0.53a 30.88 ± 0.57d 13.10 ± 0.57bc 3.00 ± 0.11ab 1.56 ± 0.47a 1.08 ± 0.24a
  1. Buffer indicates PBS pH 7.4 without addition of lipase. The data are mean of triplicate. Mean data accompanied by different superscript alphabets are significantly different (Tukey’s HSD test, p < 0.05). The assay was conducted at 37 °C for 30 min