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Table 1 Relative densities of hydrolysis spots produced by commercial lipases at pH 1–11 on P(3HB-co-4HB)

From: Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film

pH

Relative densities of hydrolysis spots produced by lipase from different sources

C. antarctica

C. rugosa

M. javanicus

Porcine pancreas

P. cepacia

P. fluorescens

R. arrhizus

R. niveus

R. oryzae

Buffer

1.00 ± 0.30a

1.00 ± 0.15a

1.00 ± 0.32a

1.00 ± 0.11a

1.00 ± 0.15a

1.00 ± 0.27a

1.00 ± 0.18a

1.00 ± 0.20a

1.00 ± 0.21a

1

0.70 ± 0.87a

0.82 ± 0.24a

1.05 ± 0.30a

0.84 ± 0.77a

0.72 ± 0.49a

1.26 ± 0.79a

5.77 ± 0.42abc

3.90 ± 0.21ab

1.19 ± 0.39a

2

1.35 ± 0.41a

0.89 ± 0.26a

1.03 ± 0.20a

0.55 ± 0.50a

0.88 ± 1.28a

1.09 ± 0.45a

5.53 ± 0.57abc

2.03 ± 0.32a

1.15 ± 0.14a

3

25.98 ± 0.30d

0.80 ± 0.11a

1.10 ± 0.55a

1.04 ± 0.47a

2.53 ± 0.15a

1.28 ± 0.91a

6.03 ± 0.53abc

6.41 ± 0.54abc

1.19 ± 0.29a

4

14.27 ± 0.20bc

4.24 ± 0.26b

0.83 ± 0.28a

0.91 ± 0.65a

15.51 ± 0.40abcd

2.29 ± 0.30ab

27.65 ± 0.49bcd

8.24 ± 0.23bcd

1.20 ± 0.20a

5

20.93 ± 0.25 cd

6.11 ± 0.09c

3.56 ± 0.42ab

0.81 ± 0.53a

19.05 ± 0.10bcd

4.36 ± 0.34ab

26.77 ± 0.59bcd

11.51 ± 0.36cde

1.20 ± 0.16a

6

30.66 ± 0.30d

7.26 ± 0.05c

3.78 ± 0.39ab

5.30 ± 0.24bc

17.47 ± 0.25abcd

4.48 ± 0.28ab

29.37 ± 0.04 cd

13.35 ± 0.13de

5.19 ± 0.60b

7

51.64 ± 0.01e

6.77 ± 0.09c

8.35 ± 0.41b

8.91 ± 0.36c

20.85 ± 0.20 cd

7.45 ± 0.48abc

23.60 ± 0.77abcd

14.79 ± 0.26e

3.77 ± 0.40ab

8

6.40 ± 0.39ab

0.83 ± 0.33a

6.53 ± 0.69b

8.58 ± 0.39c

11.45 ± 0.31abc

17.59 ± 0.30c

35.18 ± 0.22d

17.34 ± 0.03e

5.20 ± 0.15b

9

1.20 ± 0.23a

0.71 ± 0.22a

0.91 ± 0.29a

3.86 ± 0.22ab

10.15 ± 0.26abc

17.26 ± 0.25c

24.28 ± 0.20abcd

1.38 ± 0.42a

6.69 ± 0.08b

10

0.99 ± 0.27a

0.80 ± 0.13a

0.88 ± 0.55a

1.32 ± 0.41a

8.27 ± 0.02abc

18.26 ± 0.41c

3.15 ± 0.31ab

1.27 ± 0.25a

1.17 ± 0.35a

11

0.86 ± 0.38a

0.66 ± 0.30a

1.10 ± 0.62a

0.49 ± 0.53a

30.88 ± 0.57d

13.10 ± 0.57bc

3.00 ± 0.11ab

1.56 ± 0.47a

1.08 ± 0.24a

  1. Buffer indicates PBS pH 7.4 without addition of lipase. The data are mean of triplicate. Mean data accompanied by different superscript alphabets are significantly different (Tukey’s HSD test, p < 0.05). The assay was conducted at 37 °C for 30 min