Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film

Mok, Pei-Shze; Ch’ng, Diana Hooi-Ean; Ong, Soo-Peng; Numata, Keiji; Sudesh, Kumar

doi:10.1186/s13568-016-0230-z

AMB Express

Table 1 Relative densities of hydrolysis spots produced by commercial lipases at pH 1–11 on P(3HB-co-4HB)

From: Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film

pH	Relative densities of hydrolysis spots produced by lipase from different sources
pH	C. antarctica	C. rugosa	M. javanicus	Porcine pancreas	P. cepacia	P. fluorescens	R. arrhizus	R. niveus	R. oryzae
Buffer	1.00 ± 0.30^a	1.00 ± 0.15^a	1.00 ± 0.32^a	1.00 ± 0.11^a	1.00 ± 0.15^a	1.00 ± 0.27^a	1.00 ± 0.18^a	1.00 ± 0.20^a	1.00 ± 0.21^a
1	0.70 ± 0.87^a	0.82 ± 0.24^a	1.05 ± 0.30^a	0.84 ± 0.77^a	0.72 ± 0.49^a	1.26 ± 0.79^a	5.77 ± 0.42^abc	3.90 ± 0.21^ab	1.19 ± 0.39^a
2	1.35 ± 0.41^a	0.89 ± 0.26^a	1.03 ± 0.20^a	0.55 ± 0.50^a	0.88 ± 1.28^a	1.09 ± 0.45^a	5.53 ± 0.57^abc	2.03 ± 0.32^a	1.15 ± 0.14^a
3	25.98 ± 0.30^d	0.80 ± 0.11^a	1.10 ± 0.55^a	1.04 ± 0.47^a	2.53 ± 0.15^a	1.28 ± 0.91^a	6.03 ± 0.53^abc	6.41 ± 0.54^abc	1.19 ± 0.29^a
4	14.27 ± 0.20^bc	4.24 ± 0.26^b	0.83 ± 0.28^a	0.91 ± 0.65^a	15.51 ± 0.40^abcd	2.29 ± 0.30^ab	27.65 ± 0.49^bcd	8.24 ± 0.23^bcd	1.20 ± 0.20^a
5	20.93 ± 0.25 ^cd	6.11 ± 0.09^c	3.56 ± 0.42^ab	0.81 ± 0.53^a	19.05 ± 0.10^bcd	4.36 ± 0.34^ab	26.77 ± 0.59^bcd	11.51 ± 0.36^cde	1.20 ± 0.16^a
6	30.66 ± 0.30^d	7.26 ± 0.05^c	3.78 ± 0.39^ab	5.30 ± 0.24^bc	17.47 ± 0.25^abcd	4.48 ± 0.28^ab	29.37 ± 0.04 ^cd	13.35 ± 0.13^de	5.19 ± 0.60^b
7	51.64 ± 0.01^e	6.77 ± 0.09^c	8.35 ± 0.41^b	8.91 ± 0.36^c	20.85 ± 0.20 ^cd	7.45 ± 0.48^abc	23.60 ± 0.77^abcd	14.79 ± 0.26^e	3.77 ± 0.40^ab
8	6.40 ± 0.39^ab	0.83 ± 0.33^a	6.53 ± 0.69^b	8.58 ± 0.39^c	11.45 ± 0.31^abc	17.59 ± 0.30^c	35.18 ± 0.22^d	17.34 ± 0.03^e	5.20 ± 0.15^b
9	1.20 ± 0.23^a	0.71 ± 0.22^a	0.91 ± 0.29^a	3.86 ± 0.22^ab	10.15 ± 0.26^abc	17.26 ± 0.25^c	24.28 ± 0.20^abcd	1.38 ± 0.42^a	6.69 ± 0.08^b
10	0.99 ± 0.27^a	0.80 ± 0.13^a	0.88 ± 0.55^a	1.32 ± 0.41^a	8.27 ± 0.02^abc	18.26 ± 0.41^c	3.15 ± 0.31^ab	1.27 ± 0.25^a	1.17 ± 0.35^a
11	0.86 ± 0.38^a	0.66 ± 0.30^a	1.10 ± 0.62^a	0.49 ± 0.53^a	30.88 ± 0.57^d	13.10 ± 0.57^bc	3.00 ± 0.11^ab	1.56 ± 0.47^a	1.08 ± 0.24^a

Buffer indicates PBS pH 7.4 without addition of lipase. The data are mean of triplicate. Mean data accompanied by different superscript alphabets are significantly different (Tukey’s HSD test, p < 0.05). The assay was conducted at 37 °C for 30 min

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