Table 1 Kinetic parameters for recombinant MrSGT with UDP-Glc as the glycosyl donor and five different sterols as acceptors
Acceptor | Donor | k cat (min−1) | K m (D) (mM) | K m (A) (mM) | k cat/K m (A) (min−1 mM−1) |
|---|---|---|---|---|---|
β-sitosterol | UDP-Glc | 7.31 ± 1.03 | 0.28 ± 0.03 | 0.09 ± 0.01 | 81.2 |
Campesterol | UDP-Glc | 7.66 ± 0.99 | 0.31 ± 0.04 | 0.28 ± 0.03 | 27.4 |
Cholesterol | UDP-Glc | 7.90 ± 1.11 | 0.33 ± 0.06 | 0.45 ± 0.05 | 17.5 |
Fucosterol | UDP-Glc | 7.33 ± 1.64 | 0.27 ± 0.04 | 0.13 ± 0.02 | 56.4 |
Gramisterol | UDP-Glc | 7.83 ± 1.02 | 0.32 ± 0.05 | 0.51 ± 0.06 | 15.4 |