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Table 1 Kinetic parameters for recombinant MrSGT with UDP-Glc as the glycosyl donor and five different sterols as acceptors

From: Kinetic studies on recombinant UDP-glucose: sterol 3-O-β-glycosyltransferase from Micromonospora rhodorangea and its bioconversion potential

Acceptor Donor k cat (min−1) K m (D) (mM) K m (A) (mM) k cat/K m (A) (min−1 mM−1)
β-sitosterol UDP-Glc 7.31 ± 1.03 0.28 ± 0.03 0.09 ± 0.01 81.2
Campesterol UDP-Glc 7.66 ± 0.99 0.31 ± 0.04 0.28 ± 0.03 27.4
Cholesterol UDP-Glc 7.90 ± 1.11 0.33 ± 0.06 0.45 ± 0.05 17.5
Fucosterol UDP-Glc 7.33 ± 1.64 0.27 ± 0.04 0.13 ± 0.02 56.4
Gramisterol UDP-Glc 7.83 ± 1.02 0.32 ± 0.05 0.51 ± 0.06 15.4