Table 3 Taguchi OA design experiments for the selection of most contributing factors for tannase activity from Klebsiella pneumoniae using agro-residue as substrate
Run | pH | Temp | Incubation time (h) | Rpm | Inoculum level (%) | Tannin extract (%) | Glucose (%) | NH4Cl (%) | KH2PO4 (%) | K2HPO4 (%) | MgSO4 (%) | Response |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 4 | 30 | 96 | 150 | 5 | 1 | 0 | 0.1 | 0.3 | 0.3 | 0.1 | 0.0315 |
2 | 4 | 40 | 96 | 50 | 5 | 3 | 0 | 0.5 | 0.1 | 0.3 | 0.05 | 0.0330 |
3 | 6 | 30 | 48 | 150 | 5 | 3 | 0 | 0.5 | 0.3 | 0.1 | 0.05 | 0.0290 |
4 | 4 | 30 | 48 | 50 | 1 | 3 | 2 | 0.5 | 0.3 | 0.3 | 0.1 | 0.0298 |
5 | 6 | 30 | 96 | 50 | 5 | 3 | 2 | 0.1 | 0.1 | 0.1 | 0.1 | 0.0303 |
6 | 6 | 40 | 48 | 50 | 5 | 1 | 2 | 0.1 | 0.3 | 0.3 | 0.05 | 0.0295 |
7 | 6 | 40 | 96 | 50 | 1 | 1 | 0 | 0.5 | 0.3 | 0.1 | 0.1 | 0.0292 |
8 | 4 | 40 | 48 | 150 | 5 | 1 | 2 | 0.5 | 0.1 | 0.1 | 0.1 | 0.0305 |
9 | 4 | 40 | 96 | 150 | 1 | 3 | 2 | 0.1 | 0.3 | 0.1 | 0.05 | 0.0320 |
10 | 6 | 30 | 96 | 150 | 1 | 1 | 2 | 0.5 | 0.1 | 0.3 | 0.05 | 0.0281 |
11 | 4 | 30 | 48 | 50 | 1 | 1 | 0 | 0.1 | 0.1 | 0.1 | 0.05 | 0.0281 |
12 | 6 | 40 | 48 | 150 | 1 | 3 | 0 | 0.1 | 0.1 | 0.3 | 0.1 | 0.0305 |