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Table 2 Central composite design of the variables with tannase activity from Klebsiella pneumoniae as response using agro-residue as substrate

From: Improved production of tannase by Klebsiella pneumoniae using Indian gooseberry leaves under submerged fermentation using Taguchi approach

Run

pH

Incubation temp (°C)

Incubation time (h)

Tannin content (%)

Actual value

Predicted value

1

5.5{0}

37.5{0}

84{0}

0{−2}

0.0015

0.0013

2

4{−1}

25{−1}

120{1}

3{1}

0.0114

0.0113

3

5.5{0}

37.5{0}

84{0}

2{0}

0.0443

0.0416

4

7{1}

25{−1}

48{−1}

1{−1}

0.0148

0.0141

5

5.5{0}

12.5{−2}

84{0}

2{0}

0.0097

0.0123

6

5.5{0}

37.5{0}

84{0}

2{0}

0.0352

0.0416

7

5.5{0}

37.5{0}

84{0}

2{0}

0.0352

0.0416

8

5.5{0}

37.5{0}

84{0}

4{2}

0.0250

0.0243

9

4{−1}

50{1}

48{−1}

1{−1}

0.0156

0.0160

10

7{1}

50{1}

120{1}

3{1}

0.0136

0.0178

11

5.5{0}

37.5{0}

84{0}

2{0}

0.0444

0.0416

12

7{1}

50{1}

48{−1}

1{−1}

0.0151

0.0134

13

2.5{−2}

37.5{0}

84{0}

2{0}

0.0125

0.0101

14

7{1}

50{1}

48{−1}

3{1}

0.0139

0.0118

15

7{1}

25{−1}

48{−1}

3{1}

0.0173

0.0171

16

4{−1}

25{−1}

48{−1}

1{−1}

0.0012

0.0013

17

4{−1}

50{1}

120{1}

1{−1}

0.0211

0.0215

18

4{−1}

25{−1}

48{−1}

3{1}

0.0182

0.0118

19

5.5{0}

37.5{0}

84{0}

2{0}

0.0443

0.0416

20

5.5{0}

37.5{0}

84{0}

2{0}

0.0463

0.0416

21

5.5{0}

62.5{2}

84{0}

2{0}

0.0188

0.0198

22

8.5{2}

37.5{0}

84{0}

2{0}

0.0143

0.0142

23

4{−1}

25{−1}

120{1}

1{−1}

0.0074

0.0087

24

5.5{0}

37.5{0}

12{−2}

2{0}

0.0112

0.0119

25

7.5{1}

25{−1}

120{1}

3{1}

0.0241

0.0249

26

7.5{1}

25{−1}

120{1}

1{−1}

0.0183

0.0229

27

4{−1}

50{1}

120{1}

3{1}

0.0266

0.0265

28

7.5{1}

50{1}

120{1}

1{−1}

0.0277

0.0283

29

4{−1}

50{1}

48{−1}

3{1}

0.0221

0.0219

30

5.5{0}

37.5{0}

156{2}

2{0}

0.0254

0.0252