S. no. | Factor | Level 1 (−1) | Level 2 (+1) |
---|---|---|---|
1 | pH | 4 | 6 |
2 | Temperature | 30 | 40 |
3 | Incubation time (hrs) | 48 | 96 |
4 | rpm | 50 | 150 |
5 | Inoculum level (%) | 1 | 5 |
6 | Tannin extract (%) | 1 | 3 |
7 | Glucose (%) | 0 | 2 |
8 | NH4Cl (%) | 0.1 | 0.5 |
9 | KH2PO4 (%) | 0.1 | 0.3 |
10 | K2HPO4 (%) | 0.1 | 0.3 |
11 | MgSO4 (%) | 0.05 | 0.1 |