Table 2 Sequences of oligonucleotides used to construct plasmids
From: Continuous crossbreeding of sake yeasts using growth selection systems for a-type and α-type cells
Number | Sequence |
|---|---|
1 | 5′-CAAAgcggccgcATGGATAGATCCGGAAAGCC-3′ |
2 | 5′-AATTTATTTCggatccCTATTCCTTTGCCCTCGGAC-3′ |
3 | 5′-TATAGGGCGAATTGgagctcGAATAAAAAACACGCTTTTT-3′ |
4 | 5′-CATgcggccgcTTTGTTTGTTTATGTGTGTT-3′ |
5 | 5′-GCTTATGTAAggatccGAAATAAATTGAATTGAAT-3′ |
6 | 5′-CGGGCCCCCCctcgagAGCTTTAACGAACGCAGAA-3′ |
7 | 5′-AATTGGAGCTCCAccgcggATCTGTTTAGCTTGCCTCGT-3′ |
8 | 5′-CGGGCCCCCCctcgagCTCGTTTTCGACACTGGAT-3′ |
9 | 5′-CTCGAGGGGGGGCCCGgagctcAGCTTTAACGAACGCAGAA-3′ |
10 | 5′-GGTGATATTGGATaccgcggAGATGCCGATTTGGGC-3′ |
11 | 5′-CGGGCCCCCCctcgag-3′ |
12 | 5′-TTTTCAACAAAATccgcgg-3′ |
13 | 5′-CGGGCCCCCCctcgagGAGCGACCTCATGCTATA-3′ |