Table 1 Overview of probes used to target the organisms of interest for the FISH microscopic analysis of the enrichment culture
From: Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions
Reference | Probe | Sequence (5′–3′) | Target | Formamide (%) |
|---|---|---|---|---|
Amann et al. (1990) | EUB | GCTGCCTCCCGTAGGAGT | Many but not all Bacteria | 0–70 |
Daims et al. (1999) | EUB II | GCAGCCACCCGTAGGTGT | Planctomycetales | 0–50 |
Daims et al. (1999) | EUB III | GCTGCCACCCGTAGGTGT | Verrucomicrobiales | 0–50 |
Crocetti et al. (2000) | PAO 651 | CCCTCTGCCAAACTCCAG | ‘Candidatus Accumulibacter phosphatis’ | 35 |
Crocetti et al. (2002) | GAOQ431 | TCCCCGCCTAAAGGGCTT | ‘Candidatus Competibacter phosphatis’ | 35 |
Crocetti et al. (2002) | GAOQ989 | TTCCCCGGATGTCAAGGC | ‘Candidatus Competibacter phosphatis’ | 35 |
Flowers et al. (2009) | Acc-I-444 | CCCAAGCAATTTCTTCCCC | ‘Candidatus Accumulibacter phosphatis’ Clade IA and other Type I clades | 35 |
Flowers et al. (2009) | Acc-II-444 | CCCGTGCAATTTCTTCCCC | ‘Candidatus Accumulibacter phosphatis’ Clade IIA, IIC and IID | 35 |