Skip to main content

Table 1 Overview of probes used to target the organisms of interest for the FISH microscopic analysis of the enrichment culture

From: Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions

Reference Probe Sequence (5′–3′) Target Formamide (%)
Amann et al. (1990) EUB GCTGCCTCCCGTAGGAGT Many but not all Bacteria 0–70
Daims et al. (1999) EUB II GCAGCCACCCGTAGGTGT Planctomycetales 0–50
Daims et al. (1999) EUB III GCTGCCACCCGTAGGTGT Verrucomicrobiales 0–50
Crocetti et al. (2000) PAO 651 CCCTCTGCCAAACTCCAG ‘Candidatus Accumulibacter phosphatis’ 35
Crocetti et al. (2002) GAOQ431 TCCCCGCCTAAAGGGCTT ‘Candidatus Competibacter phosphatis’ 35
Crocetti et al. (2002) GAOQ989 TTCCCCGGATGTCAAGGC ‘Candidatus Competibacter phosphatis’ 35
Flowers et al. (2009) Acc-I-444 CCCAAGCAATTTCTTCCCC ‘Candidatus Accumulibacter phosphatis’ Clade IA and other Type I clades 35
Flowers et al. (2009) Acc-II-444 CCCGTGCAATTTCTTCCCC ‘Candidatus Accumulibacter phosphatis’ Clade IIA, IIC and IID 35