Fig. 2
From: Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions
Representative FISH-microscopy images (a, b) and phase contrast images (c, d) showing the distribution of bacterial populations in the enriched biomass culture. In a, blue (EUB mix, Cy5): all bacteria other than ‘Candidatus Accumulibacter phosphatis’ and ‘Candidatus Competibacter phosphatis’; purple (superposition of PAO mix 651, Cy3 and EUB mix, Cy5): ‘Candidatus Accumulibacter phosphatis’; and cyan green (superposition of GAO mix, Fluos and EUB mix, Cy5): ‘Candidatus Competibacter phosphatis’. In b, blue (PAO mix 651, Cy5): all potential PAO that do not stain with specific probes for ‘Candidatus Accumulibacter phosphatis’ type I and II, purple (superposition of PAO mix 651, Cy5 and Acc II, Cy3): ‘Candidatus Accumulibacter phosphatis’ type II, and cyan green (superposition of PAO mix 651, Cy5 and Acc I, Fluos): ‘Candidatus Accumulibacter phosphatis’ type I. In c, dark cells: bacteria with the typical ‘Candidatus Accumulibacter phosphatis’ morphology; bright cells: bacteria with the typical ‘Candidatus Competibacter phosphatis’ morphology. In d, small size: bacteria with the typical ‘Candidatus Accumulibacter phosphatis’ morphology; big size: bacteria with the typical ‘Candidatus Competibacter phosphatis’ morphology