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Table 1 Protein identification by MASCOT Search on the purified enzyme from Bacillus strain C4 using RefSeq protein data base and the two amino acid sequences retrieved from Ker1 and Ker2

From: Identification of two new keratinolytic proteases from a Bacillus pumilus strain using protein analysis and gene sequencing

SDS-Page lane Identified protein Sequence identity MW (kDa) MASCOT peptides identified MASCOT ion scorea
1 and 2 Keratinase 1 WP_008348814.1 38.8 QRLENTATPLGNSFYYGK 81
GVVVVAAAGNSGSTGSTSTVGYPAK 146
YDSTIAVANVNSNNVR 122
LENTATPLGNSFYYGK 102
GLINVQAASN 85
1 Keratinase 2 WP_017357922.1 38.3 VGVVGVAPK 61
VADENGDGYYSWIIK 80
SGTSMASPHVAGAAAVILSK 107
HPNLTNDELR 53
HPNLTNDELRER 34
1 Peptidase S8 gi|648268958 38.8 VGVVGVAPK 61
VADENGDGYYSWIIK 80
LGEPFYYGAGLVNVQK 106
SGTSMASPHVAGAAAVILSK 107
  1. aIndividual ion scores >26 indicates identity or extensive homology at 95 % level of confidence