Identification of two new keratinolytic proteases from a Bacillus pumilus strain using protein analysis and gene sequencing

AMB Express

Table 1 Protein identification by MASCOT Search on the purified enzyme from Bacillus strain C₄ using RefSeq protein data base and the two amino acid sequences retrieved from Ker1 and Ker2

SDS-Page lane	Identified protein	Sequence identity	MW (kDa)	MASCOT peptides identified	MASCOT ion score^a
1 and 2	Keratinase 1	WP_008348814.1	38.8	QRLENTATPLGNSFYYGK	81
				GVVVVAAAGNSGSTGSTSTVGYPAK	146
				YDSTIAVANVNSNNVR	122
				LENTATPLGNSFYYGK	102
				GLINVQAASN	85
1	Keratinase 2	WP_017357922.1	38.3	VGVVGVAPK	61
				VADENGDGYYSWIIK	80
				SGTSMASPHVAGAAAVILSK	107
				HPNLTNDELR	53
				HPNLTNDELRER	34
1	Peptidase S8	gi\|648268958	38.8	VGVVGVAPK	61
				VADENGDGYYSWIIK	80
				LGEPFYYGAGLVNVQK	106
				SGTSMASPHVAGAAAVILSK	107

^aIndividual ion scores >26 indicates identity or extensive homology at 95 % level of confidence