Microbial community structure of two freshwater sponges using Illumina MiSeq sequencing revealed high microbial diversity

AMB Express

Table 1 Summary of species richness estimators of the samples studied

No.	Samples	Good quality sequences	Chao1^a	Observed species^a	Shannon^a	Simpson^a	Goods coverage (%)^a
1	PSPO1	144,561	5199	3215	7.68	0.98	97
2	PSPO2	168,769	5339	3218	6.84	0.93	97
3	PSPO3	150,149	3446	2408	7.7	0.98	98
4	PWAT1	86,401	2507	1840	7.43	0.98	99
5	PWAT2	102,448	4551	2740	7.55	0.98	97
6	PWAT3	88,352	5039	3308	8.19	0.99	97
7	TSPO1	67,388	1366	930	4.42	0.82	99
8	TSPO2	103,268	1471	982	4.95	0.88	99
9	TSPO3	100,068	1662	1049	5.27	0.91	99
10	TWAT1	172,090	4120	2435	7.15	0.96	98
11	TWAT2	85,384	3645	2403	7.16	0.96	98
12	TWAT3	52,206	3651	2670	7.91	0.99	98

The alpha diversity indices were calculated with 3 % distance cut-off
^aThese values were calculated after all samples were randomly sub-sampled with 52,206 sequences per samples as lowest number of sequences were found in TWAT3 sample