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Table 3 Fatty acid composition as  % of total fatty acids (g g−1) in TAG fraction of R. opacus B4 and R. opacus PD630 cultivated in different carbon sources under nitrogen limiting conditions (C/N = 300) during 24 and 72 h

From: Rhodococcus opacus B4: a promising bacterium for production of biofuels and biobased chemicals

Strain

Substrate

Time (h)

Relative proportion of fatty acids [%, w/w]

C10:0

C12:0

C14:0

C16:0

C16:1

C17:0

C17:1

C18:0

C18:1

R. opacus B4

Glucose

0

–

6.7

7.6

41.7

–

44.1

–

–

–

24

–

1.2 ± 0.09

3.2 ± 0.69

49.7 ± 0.12

–

38.8 ± 4.29

4.2 ± 1.46

3.3 ± 0.46

4.0 ± 1.42

72

–

1.4 ± 0.42

3.1 ± 0.03

38.3 ± 1.13

3.2 ± 0.21

24.8 ± 0.32

14.4 ± 0.37

2.0 ± 0.01

13.2 ± 0.26

Acetate

0

–

15.8

16.5

26.3

16.7

–

–

–

28.4

24

–

–

2.9 ± 1.17

48.6 ± 1.75

–

47.6 ± 1.28

–

–

3.4 ± 1.83

72

–

–

5.3 ± 0.57

41.1 ± 4.02

–

48.1 ± 3.42

–

–

3.9 ± 0.16

Hexadecane

0

–

1.1

5.4

70.2

16.2

–

–

–

7.8

24

27.4 ± 0.8

1.3 ± 0.08

5.7 ± 0.17

54.0 ± 1.12

9.5 ± 0.29

–

–

–

2.0 ± 0.21

72

28.9 ± 1.36

1.1 ± 0.51

6.1 ± 0.41

55.0 ± 5.1

6.8 ± 0.14

–

–

–

2.1 ± 0.68

R. opacus PD630

Glucose

0

–

–

4.2

48.9

2.9

31.4

5.2

5.1

2.0

24

–

–

2.8 ± 0.52

48.4 ± 4.43

6.1 ± 2.7

18.7 ± 1.25

12.7 ± 0.43

3.1 ± 0.09

8.2 ± 3.08

72

–

–

2.0 ± 0.04

35.3 ± 2.73

6.3 ± 2.44

16.3 ± 1.00

11.9 ± 2.31

2.5 ± 0.28

25.7 ± 5.6

Acetate

0

–

–

7.3

68.6

–

24.3

–

–

–

24

–

–

3.8 ± 0.19

55.1 ± 6.72

–

36.7 ± 1.58

8.6 ± 1.16

–

–

72

–

–

4.5 ± 0.47

55.9 ± 2.92

–

36.1 ± 1.97

6.5 ± 1.4

–

–

Hexadecane

0

–

–

28.4

69.2

–

–

–

–

–

24

–

–

13.3 ± 3.91

86.7 ± 3.91

–

–

–

–

–

72

–

–

7.3 ± 2.86

70.4 ± 7.79

19.8 ± 7.12

–

–

–

2.4 ± 0.39

  1. The means are the result of at least two independent experiments, ± s.d
  2. aCorresponds to one replicate
  3. – Not detected or <0.5 %