Rhodococcus opacus B4: a promising bacterium for production of biofuels and biobased chemicals

AMB Express

Table 1 Cell dry matter (CDW), triacylglycerol (TAG) content, % of substrate degradation and TAG yields in cells of R. opacus B4 and R. opacus PD630

Strain	Carbon source	Time (h)	CDW (g L⁻¹)	TAG (g g⁻¹ CDW)	Substrate consumed (%)	TAG yield (g g⁻¹ substrate consumed)
R. opacus B4	Glucose	0	2.2^a	0.044^a
		24	2.5 ± 0.04	0.230 ± 0.045	8.1 ± 4.3	0.203 ± 0.068
		72	3.3 ± 0.01	0.217 ± 0.014	37.4 ± 5.6	0.054 ± 0.015
	Acetate	0	0.45^a	0.081^a
		24	1.1 ± 0.1	0.252 ± 0.040	97.3 ± 0.5	0.054 ± 0.005
		72	0.724 ± 0.017	0.117 ± 0.061	100.0 ± 0.0	0.016 ± 0.002
	Hexadecane	0	0.398^a	0.079^a
		24	0.459 ± 0.134	0.186 ± 0.021	70.5 ± 5.1	0.147 ± 0.069
		72	0.837 ± 0.281	0.170 ± 0.034	85.2 ± 17.7	0.194 ± 0.112
R. opacus PD630	Glucose	0	2.4^a	0.111^a
		24	3.9 ± 0.4	0.287 ± 0.003	22.8 ± 0.8	0.076 ± 0.001
		72	4.1 ± 0.5	0.384 ± 0.051	26.5 ± 5.8	0.125 ± 0.024
	Acetate	0	0.620^a	0.169^a
		24	1.1 ± 0.01	0.246 ± 0.015	81.1 ± 10.4	0.054 ± 0.003
		72	0.726 ± 0.040	0.444 ± 0.179	100.0 ± 0.0	0.059 ± 0.033
	Hexadecane	0	0.250^a	0.063^a
		24	0.321 ± 0.168	0.141 ± 0.013	52.6 ± 9.8	0.070 ± 0.049
		72	0.468 ± 0.062	0.092 ± 0.008	94.5 ± 4.2	0.037 ± 0.004

Cells were cultivated in different carbon sources under nitrogen limiting conditions (C/N = 300) during 24 and 72 h
The means are the result of at least two independent experiments, ± standard deviation (s.d.)
^aCorresponds to one replicate