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Table 4 Differential expression of proteins associated with hydrogenase activity in C.necator H16 grown in REG glycerol bottom in comparison to REG-FFA and REG80 glycerol cultures after 24 h pi

From: Global changes in the proteome of Cupriavidus necator H16 during poly-(3-hydroxybutyrate) synthesis from various biodiesel by-product substrates

Accession #

Protein

Pnet scores

GB-24/R80-24

FFA-24/R80-24

GB-24/FFA-24

PHG002

Membrane bound (NiFe) hydrogenase large unit

−4.04

−4.96

ND

PHG019

Hydrogenase transcriptional regulatory protein, HoxA

−1.53

−1.79

ND

PHG016

[NiFe] hydrogenase metallocenter assembly protein HypD1

−1.79

−2.13

ND

PHG021

Regulatory [NiFe] hydrogenase large subunit

−1.98

−3.13

−0.84

PHG088

NAD-reducing hydrogenase diaphorase moiety large

−4.55

−5.59

ND

PHG095

[NiFe] hydrogenase nickel incorporation-associated protein HypB2

−2.06

−2.53

ND

PHG013

[NiFe] hydrogenase nickel incorporation-associated protein HypB1

−3.23

−4.99

−0.36

PHG091

NAD-reducing hydrogenase hydrogenase moiety

−4.43

−5.56

ND

PHG_00093

HoxI

−6.79

−5.39

−1.07

  1. Up- or down-regulation is limited to outer 10 % proteins with a relative Z-score expression ratio (Pnet) outside (> ±1.65) from the population mean; italic numbers indicate significant up or down regulation of protein expression