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Table 3 Differential protein expression levels (Pnet) associated with poly-(3-hydroxybutyrate) synthesis and other accessory proteins of PHB granules in C. necator H16 cultured with different substrates

From: Global changes in the proteome of Cupriavidus necator H16 during poly-(3-hydroxybutyrate) synthesis from various biodiesel by-product substrates

Locus tag Protein GB-24/R80-24 GB-24/FFA-24 FFA-24/R80-24
H16_A1437 Poly(3-hydroxybutyrate) polymerase PhaC 0.35 −0.41 −0.14
H16_A1438 Acetyl-CoA acetyltransferase PhaA −0.07 0.08 −0.15
H16_A1439 Acetoacetyl-CoA reductase PhaB −0.21 −0.01 −0.04
H16_A1440 transcriptional regulator of phasin expression PhaR 0.26 −0.29 −0.35
H16_A1381 Phasin (PHA-granule associated protein) PhaP1 2.96* 0.27 2.68
PHG202 Phasin (PHA-granule associated protein) PhaP2 ND ND ND
H16_A2172 Phasin (PHA-granule associated protein) PhaP3 ND ND ND
H16_B2021 Phasin (PHA-granule associated protein) PhaP4 −1.84 −0.58 0.88
H16_B1934 Phasin (PHA-granule associated protein) PhaP5 −0.06 ND −0.51
H16_A2862 Intracellular poly(3-hydroxybutyrate) depolymerase ND 0.34 ND
H16_A1150 Poly(3-hydroxybutyrate) depolymerase 0.27 −0.02 0.41
H16_B0339 Poly(3-hydroxybutyrate) depolymerase ND ND ND
H16_B1014 Poly(3-hydroxybutyrate) depolymerase ND ND ND
H16_B2073 Poly(3-hydroxybutyrate) depolymerase ND ND ND
H16_B2401 Poly(3-hydroxybutyrate) depolymerase ND ND ND
H16_A0141 Membrane associated protein PhaM −1.39 −0.62 −0.49
  1. Up- or down-regulation is limited to outer 10 % proteins with a relative Z-score expression ratio (Znet) outside (> ±1.65) from the population mean; italic numbers indicate significant up- or down-regulation of protein expression