Table 3 Differential protein expression levels (Pnet) associated with poly-(3-hydroxybutyrate) synthesis and other accessory proteins of PHB granules in C. necator H16 cultured with different substrates
Locus tag | Protein | GB-24/R80-24 | GB-24/FFA-24 | FFA-24/R80-24 |
|---|---|---|---|---|
H16_A1437 | Poly(3-hydroxybutyrate) polymerase PhaC | 0.35 | −0.41 | −0.14 |
H16_A1438 | Acetyl-CoA acetyltransferase PhaA | −0.07 | 0.08 | −0.15 |
H16_A1439 | Acetoacetyl-CoA reductase PhaB | −0.21 | −0.01 | −0.04 |
H16_A1440 | transcriptional regulator of phasin expression PhaR | 0.26 | −0.29 | −0.35 |
H16_A1381 | Phasin (PHA-granule associated protein) PhaP1 | 2.96* | 0.27 | 2.68 |
PHG202 | Phasin (PHA-granule associated protein) PhaP2 | ND | ND | ND |
H16_A2172 | Phasin (PHA-granule associated protein) PhaP3 | ND | ND | ND |
H16_B2021 | Phasin (PHA-granule associated protein) PhaP4 | −1.84 | −0.58 | 0.88 |
H16_B1934 | Phasin (PHA-granule associated protein) PhaP5 | −0.06 | ND | −0.51 |
H16_A2862 | Intracellular poly(3-hydroxybutyrate) depolymerase | ND | 0.34 | ND |
H16_A1150 | Poly(3-hydroxybutyrate) depolymerase | 0.27 | −0.02 | 0.41 |
H16_B0339 | Poly(3-hydroxybutyrate) depolymerase | ND | ND | ND |
H16_B1014 | Poly(3-hydroxybutyrate) depolymerase | ND | ND | ND |
H16_B2073 | Poly(3-hydroxybutyrate) depolymerase | ND | ND | ND |
H16_B2401 | Poly(3-hydroxybutyrate) depolymerase | ND | ND | ND |
H16_A0141 | Membrane associated protein PhaM | −1.39 | −0.62 | −0.49 |