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Table 3 Cloning of different fungal β-Xylosidase genes in fungi

From: Molecular cloning and comparative sequence analysis of fungal β-Xylosidases

Source of gene Gene Vector Host Molecular mass of the recombinant enzyme (kDa) Characteristics of the recombinant enzyme Reference
A. niger GS1 xlnD pGEM-T A. niger AB4.1 90 Temperature = 70 °C pH = 3.6 enzyme activity = 4280 Umg protein−1 half-life = 74 min at 70 °C activation energy = 58.9 kJ mol−1 Amaro-Reyes et al. (2011)
T. reesei RutC-30 bxl1 pAJ401 S. cerevisiae DBY746 49.1 Enzyme activity = 16.3 nkat mL−1 Margolles-Clark et al. (1996)
A. niger 90,196 xlnD pDF1 S. cerevisiae Y294 85.1 Temperature = 60 °C pH = 3.2 stability = 50 °C enzyme activity = 5.3 nkat mL−1 Grange et al. (2001)
A. japonicas MU-2 xylA pPIC9 P. pastoris GS115 113.2 Temperature = 70 °C pH = 4.0 specific activity = 112 U mg−1 km = 0.314 mM Vmax = 114 µmol mg−1 min−1 stability 60 °C for 30 min Wakiyama et al. (2008)
H. insolens Y1 xyl3A pPIC9 P. pastoris GS115 83.2 Temperature = 60 °C pH = 6 specific activity = 11.6 U mg−1 Km = 2.51 mM Vmax = 37.33 μmol min−1 mg−1 Xia et al. (2015)
T. lanuginosus SSBP tlxyn1 pBGPI P. pastoris GS115 52.3 Temperature = 50 °C pH = 7 specific activity = 2.29 U mg−1 Gramany et al. (2015)