Molecular cloning and comparative sequence analysis of fungal β-Xylosidases

AMB Express

Table 3 Cloning of different fungal β-Xylosidase genes in fungi

Source of gene	Gene	Vector	Host	Molecular mass of the recombinant enzyme (kDa)	Characteristics of the recombinant enzyme	Reference
A. niger GS1	xlnD	pGEM-T	A. niger AB4.1	90	Temperature = 70 °C pH = 3.6 enzyme activity = 4280 Umg protein⁻¹ half-life = 74 min at 70 °C activation energy = 58.9 kJ mol⁻¹	Amaro-Reyes et al. (2011)
T. reesei RutC-30	bxl1	pAJ401	S. cerevisiae DBY746	49.1	Enzyme activity = 16.3 nkat mL⁻¹	Margolles-Clark et al. (1996)
A. niger 90,196	xlnD	pDF1	S. cerevisiae Y294	85.1	Temperature = 60 °C pH = 3.2 stability = 50 °C enzyme activity = 5.3 nkat mL⁻¹	Grange et al. (2001)
A. japonicas MU-2	xylA	pPIC9	P. pastoris GS115	113.2	Temperature = 70 °C pH = 4.0 specific activity = 112 U mg^−1 km = 0.314 mM Vmax = 114 µmol mg⁻¹ min⁻¹ stability 60 °C for 30 min	Wakiyama et al. (2008)
H. insolens Y1	xyl3A	pPIC9	P. pastoris GS115	83.2	Temperature = 60 °C pH = 6 specific activity = 11.6 U mg⁻¹ K_m = 2.51 mM V_max = 37.33 μmol min⁻¹ mg⁻¹	Xia et al. (2015)
T. lanuginosus SSBP	tlxyn1	pBGPI	P. pastoris GS115	52.3	Temperature = 50 °C pH = 7 specific activity = 2.29 U mg⁻¹	Gramany et al. (2015)