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Table 2 Cloning of different fungal β-Xylosidase genes in E. coli

From: Molecular cloning and comparative sequence analysis of fungal β-Xylosidases

Source organism Gene Vector Host Molecular mass of the recombinant enzyme (kDa) Characteristics of the recombinant enzyme Reference
Acremonium cellulolyticus Y-94 bxy3A pLD10 E. coli DH 5α   Enzyme activity = 14.75 U L−1 Kanna et al. (2011)
T. reesei RutC-30 bxl1 pAJ401 E.coli JS4 49.1 Enzyme activity = 16.3 nkat mL−1 Margolles-Clark et al. (1996)
A. niger 90,196 xlnD pGEM-T Easy E. coli XL1-Blue MRF 85.1 Temperature = 60 °C pH = 3.2 enzyme activity = 5.3 nkat mL−1 stability = 50 °C Grange et al. (2001)
A. nidulans G191 xlnD pGW635 E. coli LE392 E. coli DH5α 85   Perez-Gonzalez et al. (1998)
A. niger GS1 xlnD pAN52.1 E. coli JM109 90 Temperature = 70 °C pH = 3.6 enzyme activity = 4280 Umg protein−1 half-life = 74 min at 70 °C Activation energy = 58.9 kJ mol−1 Amaro-Reyes et al. (2011)
A. oryzae RIB40 xylB pET32b E. coli DH5α, Rosetta-gami™ (DE3) pLysS 37.4 Temperature = 30 °C pH = 7 specific activity = 6.1 U/mg Km = 0.48 mM Vmax = 42.6 μmol min−1 mg−1 Suzuki et al. (2010)
H. insolens Y1 Xyl43A pEASY-T3 E. coli Trans1-T1 37 Temperature = 50 °C pH = 6.5 specific activity = 20.5 U mg−1Km = 12.2 mM Vmax = 203.8 μmol min−1 mg−1 Yang et al. (2014)
H. insolens Y1 Xyl43B pEASY-T3 E. coli Trans1-T1 62 Temperature = 50 °C pH = 7 specific activity = 1.7 U/mg Km = 1.29 mM Vmax = 2.18 μmol min−1 mg−1 Yang et al. (2014)
T. lanuginosus CAU44 TlXyl43 pET28a(+) E. coli BL21 (DE3) 51.6 Temperature = 55 °C pH = 6.5 specific activity = 45.4 U mg−1Km = 3.9 mM Vmax = 107.6 μmol min−1 mg−1 Chen et al. (2012a)
P. thermophile J18 PtXyl43 pMD-18 T E. coli BL21 (DE3) 52.3 Temperature = 55 °C pH = 7 specific activity = 45.4 U mg−1 Km = 4.5 mM Vmax = 90.2 μmol min−1 mg−1 Teng et al. (2011)