Table 2 Cloning of different fungal β-Xylosidase genes in E. coli

Acremonium cellulolyticus Y-94

bxy3A

pLD10

E. coli DH 5α

Enzyme activity = 14.75 U L⁻¹

Kanna et al. (2011)

T. reesei RutC-30

bxl1

pAJ401

E.coli JS4

Enzyme activity = 16.3 nkat mL⁻¹

Margolles-Clark et al. (1996)

A. niger 90,196

xlnD

pGEM-T Easy

E. coli XL1-Blue MRF

Temperature = 60 °C pH = 3.2 enzyme activity = 5.3 nkat mL⁻¹ stability = 50 °C

Grange et al. (2001)

A. nidulans G191

xlnD

pGW635

E. coli LE392 E. coli DH5α

Perez-Gonzalez et al. (1998)

A. niger GS1

xlnD

pAN52.1

E. coli JM109

Temperature = 70 °C pH = 3.6 enzyme activity = 4280 Umg protein⁻¹ half-life = 74 min at 70 °C Activation energy = 58.9 kJ mol⁻¹

Amaro-Reyes et al. (2011)

A. oryzae RIB40

xylB

pET32b

E. coli DH5α, Rosetta-gami™ (DE3) pLysS

Temperature = 30 °C pH = 7 specific activity = 6.1 U/mg K_m = 0.48 mM V_max = 42.6 μmol min⁻¹ mg⁻¹

Suzuki et al. (2010)

H. insolens Y1

Xyl43A

pEASY-T3

E. coli Trans1-T1

Temperature = 50 °C pH = 6.5 specific activity = 20.5 U mg⁻¹K_m = 12.2 mM V_max = 203.8 μmol min⁻¹ mg⁻¹

Yang et al. (2014)

H. insolens Y1

Xyl43B

pEASY-T3

E. coli Trans1-T1

Temperature = 50 °C pH = 7 specific activity = 1.7 U/mg K_m = 1.29 mM V_max = 2.18 μmol min⁻¹ mg⁻¹

Yang et al. (2014)

T. lanuginosus CAU44

TlXyl43

pET28a(+)

E. coli BL21 (DE3)

Temperature = 55 °C pH = 6.5 specific activity = 45.4 U mg⁻¹K_m = 3.9 mM V_max = 107.6 μmol min⁻¹ mg⁻¹

Chen et al. (2012a)

P. thermophile J18

PtXyl43

pMD-18 T

E. coli BL21 (DE3)

Temperature = 55 °C pH = 7 specific activity = 45.4 U mg⁻¹ K_m = 4.5 mM V_max = 90.2 μmol min⁻¹ mg⁻¹

Teng et al. (2011)