Fig. 3

Her2-S-Fab induces NK cell-mediated cytotoxicity. (a) Cytotoxicity assay test was performed as described in the “Materials and methods” section for cell lines CHO and SKOV3 with 10 μg/ml of Her2-Fab, Her2-S-Fab or Transtuzumab. The data are the mean of triplicates with error bars representing the standard deviation. Dark, CHO without NK cells; grey, CHO added NK; dense stripes, SKOV3 without NK cells; thin stripes, SKOV3 added NK cells. b–f dose dependent cytotoxicity assays were performed as described in the “Materials and methods” section for different cell lines, CHO (b), MDAMB435 (c), MCF7 (d), SKBR3 (e), and SKOV3 (f). The concentration of Her2-Fab, Her2-S-Fab or Transtuzumab is from 0.01 ng/ml to 10ug/ml. The data are the mean of triplicates with error bars representing the standard deviation. Solid circle, Fab without NK; solid square, Her2-S-Fab without NK; solid upright triangle, Transtuzumab without NK; solid inverted triangle, Fab added NK; solid diamond, Her2-S-Fab added NK; open circle, Transtuzumab added NK. NK cells (25,000 per well) and effector cells (2500 per well). The mixtures were incubated for 72 h before cytotoxicity measurement