Table 1 Bacterial strains and plasmids used in this study
From: Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli
Strain or plasmid | Relevant marker | Source or reference |
|---|---|---|
Escherichia coli | ||
JM109 | F′ traD36 proA + B + lacI qΔ(lacZ)M15/Δ(lac-proAB) glnV44 e14 − gyrA96 recA1 relA1 endA1 thi hsdR17 | Invitrogen |
Plasmids | ||
pBBR-CAB | pBBR1-MCS2 derivative, phaCAB | (Ushimaru et al. 2014) |
pTrcHis2B | pBR322 ori, P trc , lacI q, Ampr | Invitrogen |
pTrc-pct | pTrcHis2B derivative, pct | This study |
pTrc-pct-bktB | pTrcHis2B derivative, pct, bktB | This study |