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Table 1 Bacterial strains and plasmids used in this study

From: Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli

Strain or plasmid Relevant marker Source or reference
Escherichia coli
 JM109 F′ traD36 proA + B + lacI qΔ(lacZ)M15/Δ(lac-proAB) glnV44 e14 gyrA96 recA1 relA1 endA1 thi hsdR17 Invitrogen
Plasmids
 pBBR-CAB pBBR1-MCS2 derivative, phaCAB (Ushimaru et al. 2014)
 pTrcHis2B pBR322 ori, P trc , lacI q, Ampr Invitrogen
 pTrc-pct pTrcHis2B derivative, pct This study
 pTrc-pct-bktB pTrcHis2B derivative, pct, bktB This study