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Table 3 Plackett–Burman experimental design matrix PB12 (real and coded values) with their respective responses (enzymatic activity)

From: Marine-derived fungus Aspergillus cf. tubingensis LAMAI 31: a new genetic resource for xylanase production

Run Variablesa,b Inoculum (pellets) pH Xylanase (U/mL)
Peptone RS Xylan
1 40 (1) 30 (−1) 40 (1) 3 (−1) 6 (−1) 104.48
2 40 (1) 90 (1) 0 (−1) 5 (1) 6 (−1) 629.67
3 0 (−1) 90 (1) 40 (1) 3 (−1) 8 (1) 78.36
4 40 (1) 30 (−1) 40 (1) 5 (1) 6 (−1) 496.26
5 40 (1) 90 (1) 0 (−1) 5 (1) 8 (1) 609.78
6 40 (1) 90 (1) 40 (1) 3 (−1) 8 (1) 180.24
7 0 (−1) 90 (1) 40 (1) 5 (1) 6 (−1) 71.27
8 0 (−1) 30 (−1) 40 (1) 5 (1) 8 (1) 18.58
9 0 (−1) 30 (−1) 0 (−1) 5 (1) 8 (1) 26.64
10 40 (1) 30 (−1) 0 (−1) 3 (−1) 8 (1) 93.66
11 0 (−1) 90 (1) 0 (−1) 3 (−1) 6 (−1) 129.67
12 0 (−1) 30 (−1) 0 (−1) 3 (−1) 6 (−1) 139.75
13 (C) 20 (0) 60 (0) 20 (0) 4 (0) 7 (0) 358.53
14 (C) 20 (0) 60 (0) 20 (0) 4 (0) 7 (0) 421.82
15 (C) 20 (0) 60 (0) 20 (0) 4 (0) 7 (0) 439.13
16 (C) 20 (0) 60 (0) 20 (0) 4 (0) 7 (0) 439.43
  1. The experiments were conducted at 28 °C and 140 rpm
  2. c central point, RS rice straw
  3. a In parenthesis coded values
  4. b Values in g/L
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