Table 3 Plackett–Burman experimental design matrix PB12 (real and coded values) with their respective responses (enzymatic activity)
Run | Variablesa,b | Inoculum (pellets) | pH | Xylanase (U/mL) | ||
|---|---|---|---|---|---|---|
Peptone | RS | Xylan | ||||
1 | 40 (1) | 30 (−1) | 40 (1) | 3 (−1) | 6 (−1) | 104.48 |
2 | 40 (1) | 90 (1) | 0 (−1) | 5 (1) | 6 (−1) | 629.67 |
3 | 0 (−1) | 90 (1) | 40 (1) | 3 (−1) | 8 (1) | 78.36 |
4 | 40 (1) | 30 (−1) | 40 (1) | 5 (1) | 6 (−1) | 496.26 |
5 | 40 (1) | 90 (1) | 0 (−1) | 5 (1) | 8 (1) | 609.78 |
6 | 40 (1) | 90 (1) | 40 (1) | 3 (−1) | 8 (1) | 180.24 |
7 | 0 (−1) | 90 (1) | 40 (1) | 5 (1) | 6 (−1) | 71.27 |
8 | 0 (−1) | 30 (−1) | 40 (1) | 5 (1) | 8 (1) | 18.58 |
9 | 0 (−1) | 30 (−1) | 0 (−1) | 5 (1) | 8 (1) | 26.64 |
10 | 40 (1) | 30 (−1) | 0 (−1) | 3 (−1) | 8 (1) | 93.66 |
11 | 0 (−1) | 90 (1) | 0 (−1) | 3 (−1) | 6 (−1) | 129.67 |
12 | 0 (−1) | 30 (−1) | 0 (−1) | 3 (−1) | 6 (−1) | 139.75 |
13 (C) | 20 (0) | 60 (0) | 20 (0) | 4 (0) | 7 (0) | 358.53 |
14 (C) | 20 (0) | 60 (0) | 20 (0) | 4 (0) | 7 (0) | 421.82 |
15 (C) | 20 (0) | 60 (0) | 20 (0) | 4 (0) | 7 (0) | 439.13 |
16 (C) | 20 (0) | 60 (0) | 20 (0) | 4 (0) | 7 (0) | 439.43 |