Run
|
pH
|
ASW (%)
|
Variablesa,b
|
Inoculum (pellet)
|
Xylanase (U/mL)
|
---|
(NH4)2SO4
|
Peptone
|
SB
|
WB
|
RS
|
Sucrose
|
Xilan
|
---|
1
|
7 (+1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
60 (+1)
|
0 (−1)
|
0 (−1)
|
20 (+1)
|
0 (−1)
|
3 (+1)
|
16.1
|
2
|
7 (+1)
|
100 (+1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
60 (+1)
|
0 (−1)
|
0 (−1)
|
20 (+1)
|
3 (+1)
|
45.1
|
3
|
7 (+1)
|
100 (+1)
|
4 (+1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
60 (+1)
|
0 (−1)
|
20 (+1)
|
1 (−1)
|
1.8
|
4
|
7 (+1)
|
100 (+1)
|
4 (+1)
|
20 (+1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
20 (+1)
|
0 (−1)
|
1 (−1)
|
2.4
|
5
|
5 (−1)
|
100 (+1)
|
4 (+1)
|
20 (+1)
|
60 (+1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
3 (+1)
|
0
|
6
|
7 +1)
|
0 (−1)
|
4 (+1)
|
20 (+1)
|
60 (+1)
|
60 (+1)
|
0 (−1)
|
0 (−1)
|
20 (+1)
|
1 (−1)
|
494.4
|
7
|
5(−1)
|
100 (+1)
|
0 (−1)
|
20 (+1)
|
60 (+1)
|
60 (+1)
|
60 (+1)
|
0 (−1)
|
0 (−1)
|
1 (−1)
|
109.3
|
8
|
7 (+1)
|
0 (−1)
|
4 (+1)
|
0 (−1)
|
60 (+1)
|
60 (+1)
|
60 (+1)
|
20 (+1)
|
0 (−1)
|
1 (−1)
|
290.5
|
9
|
7 (+1)
|
100 (+1)
|
0 (−1)
|
20 (+1)
|
0 (−1)
|
60 (+1)
|
60 (+1)
|
20 (+1)
|
0 (−1)
|
3 (+1)
|
38.3
|
10
|
5 (−1)
|
100 (+1)
|
4 (+1)
|
0 (−1)
|
60 (+1)
|
0 (−1)
|
60 (+1)
|
20 (+1)
|
20 (+1)
|
3 (+1)
|
15.3
|
11
|
5 (−1)
|
0 (−1)
|
4 (+1)
|
20 (+1)
|
0 (−1)
|
60 (+1)
|
0 (−1)
|
20 (+1)
|
20 (+1)
|
3 (+1)
|
83.1
|
12
|
7 (+1)
|
0 (−1)
|
0 (−1)
|
20 (+1)
|
60 (+1)
|
0 (−1)
|
60 (+1)
|
0 (−1)
|
20 (+1)
|
3 (+1)
|
501.9
|
13
|
5 (−1)
|
100 (+1)
|
0 (−1)
|
0 (−1)
|
60 (+1)
|
60 (+1)
|
0 (−1)
|
20 (+1)
|
20 (+1)
|
1 (−1)
|
51.3
|
14
|
5 (−1)
|
0 (−1)
|
4 (+1)
|
0 (−1)
|
0 (−1)
|
60 (+1)
|
60 (+1)
|
0 (−1)
|
0 (−1)
|
3 (+1)
|
50.3
|
15
|
5 (−1)
|
0 (−1)
|
0 (−1)
|
20 (+1)
|
0 (−1)
|
0 (−1)
|
60 (+1)
|
20 (+1)
|
20 (+1)
|
1 (−1)
|
86.5
|
16
|
5 (−1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
0 (−1)
|
1 (−1)
|
0
|
17 (C)
|
6 (0)
|
50 (0)
|
2 (0)
|
10 (0)
|
30 (0)
|
30 (0)
|
30 (0)
|
10 (0)
|
10 (0)
|
2 (0)
|
370.2
|
18 (C)
|
6 (0)
|
50 (0)
|
2 (0)
|
10 (0)
|
30 (0)
|
30 (0)
|
30 (0)
|
10 (0)
|
10 (0)
|
2 (0)
|
246.7
|
19 (C)
|
6 (0)
|
50 (0)
|
2 (0)
|
10 (0)
|
30 (0)
|
30 (0)
|
30 (0)
|
10 (0)
|
10 (0)
|
2 (0)
|
319.8
|
20 (C)
|
6 (0)
|
50 (0)
|
2 (0)
|
10 (0)
|
30 (0)
|
30 (0)
|
30 (0)
|
10 (0)
|
10 (0)
|
2 (0)
|
381.0
|
- The experiments were conducted at 28 °C and 140 rpm
-
c central point, SB sugarcane bagasse, WB wheat bran, RS rice straw
-
a In parenthesis coded values
-
b Plackett–Burman values in g/L