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Table 4 Regression summary for full polynomial equation for evaluation of siderophore production using PBD

From: Unusual non-fluorescent broad spectrum siderophore activity (SID EGYII) by Pseudomonas aeruginosa strain EGYII DSM 101801 and a new insight towards simple siderophore bioassay

Independent variable Coefficient symbol Β coefficient P value t value % confidence
Intercept B0 274 4.7e−11 29.46 99.99
Incubation time (X1, hours) B1 2.0 0.8300 0.22
Glucose (X2, w/v %) B2 −6 0.5300 −0.65
pH (X3) B3 15 0.1400 1.6
Incubation temperature (X4, °C) B4 −6 0.5300 −0.65
Agitation speed (X5, rpm) B5 22 0.0390 2.37 96.1
Glycerol (X6, w/v %) B6 −18 0.0800 −1.94
Starch (X7, w/v %) B7 −55 0.0002 −5.9 99.98
Inoculum size (X8, v/v %) B8 3 0.7500 0.32
Yeast extract (X9, g/L) B9 14 0.1600 1.51
  1. * Significant P < 0.05 and R2 = 0.83 and Adjusted R2 = 0.68 and P value for the model = 0.007