Unusual non-fluorescent broad spectrum siderophore activity (SID EGYII) by Pseudomonas aeruginosa strain EGYII DSM 101801 and a new insight towards simple siderophore bioassay

AMB Express

Table 4 Regression summary for full polynomial equation for evaluation of siderophore production using PBD

Independent variable	Coefficient symbol	Β coefficient	P value	t value	% confidence
Intercept	B0	274	4.7e−11	29.46	99.99
Incubation time (X₁, hours)	B1	2.0	0.8300	0.22	–
Glucose (X₂, w/v %)	B2	−6	0.5300	−0.65	–
pH (X₃)	B3	15	0.1400	1.6	–
Incubation temperature (X₄, °C)	B4	−6	0.5300	−0.65	–
Agitation speed (X₅, rpm)	B5	22	0.0390	2.37	96.1
Glycerol (X₆, w/v %)	B6	−18	0.0800	−1.94	–
Starch (X₇, w/v %)	B7	−55	0.0002	−5.9	99.98
Inoculum size (X₈, v/v %)	B8	3	0.7500	0.32	–
Yeast extract (X₉, g/L)	B9	14	0.1600	1.51	–

* Significant P < 0.05 and R² = 0.83 and Adjusted R² = 0.68 and P value for the model = 0.007