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Table 2 Morphological, biochemical profile and antibiotic sensitivity tests of the bacterial isolate S33

From: Unusual non-fluorescent broad spectrum siderophore activity (SID EGYII) by Pseudomonas aeruginosa strain EGYII DSM 101801 and a new insight towards simple siderophore bioassay

Test

Result

Cell shape

Rods

Gram stain

−ve

Spore formation

−ve

Growth on MYP

Non-mannitol utilizer

Motility

−ve

Fluorescence on Pseudomonas base agar (modified King A)

Yellowish green pyoverdin fluorescence under UV (fluorescein)

Growth at pH 6–8

+ve

Growth at 42 °C

+ve

Growth at 7.5 % NaCl

−ve

Starch hydrolysis

−ve

Casein hydrolysis

+ve

Tween 20 hydrolysis

−ve

Nitrate reduction

+ve

Catalase

+ve

Oxidase

+ve

Urease

+ve

Indole production

−ve

Citrate utilization

+ve

Voges–Proskauer (VP)

−ve

Methyl Red (MR)

+ve

Gelatin liquefaction

−ve

Hemolysis on sheep blood agar

+ve β-hemolytic

Glucose fermentation

+ve (yellow/blue: O/NF)

Galactose fermentation

+ve (yellow)

Sucrose fermentation

ND

Arginine utilization

−ve

Antibiotic sensitivity

 Kanamycin

Sensitive

 Penicillin

Resistant

 Ampicillin

Resistant

 Chloramphenicol

Sensitive

 TOB10

Sensitive

 Tetracycline

Resistant

  1. O/NF oxidative/non-fermentative, ND not determined resistant
  2. −ve negative result, +ve positive result