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Table 1 PBD showing levels of nine tested independent variables along with the response (SID EGY II activity)

From: Unusual non-fluorescent broad spectrum siderophore activity (SID EGYII) by Pseudomonas aeruginosa strain EGYII DSM 101801 and a new insight towards simple siderophore bioassay

Trial# Independent variable Dependent variable (Y) SID EGY II activity (SAU/mL)
X1 X2 X3 X4 X5 X6 X7 X8 X9 Exp Pred
1 −1(24) 1(1) 1(7.2) −1(25) −1(120) −1(0) −1(0) 1(12) −1(0.3) 280 327
2 1(48) −1(0.5) 1(7.2) 1(28) −1(120) −1(0) −1(0) −1(10) 1(0.5) 330 353
3 1(48) −1(0.5) −1(6.2) 1(25) −1(120) 1(1) −1(0) 1(12) −1(0.3) 290 277
4 1(48) 1(1) 1(7.2) 1(28) −1(120) −1(0) 1(1) 1(12) −1(0.3) 250 209
5 −1(24) −1(0.5) 1(7.2) −1(25) 1(180) −1(0) 1(1) 1(12) 1(0.5) 340 301
6 −1(24) 1(1) 1(7.2) 1(28) 1(180) −1(0) −1(0) 1(12) 1(0.5) 390 387
7 −1(24) −1(0.5) 1(7.2) 1(28) −1(120) 1(1) 1(1) −1(10) −1(0.3) 230 175
8 1(48) 1(1) −1(6.2) 1(28) 1(180) −1(0) −1(0) −1(10) −1(.03) 340 327
9 −1(24) 1(1) −1(7.2) −1(25) 1(180) 1(1) −1(0) −1(10) −1(0.3) 350 329
10 1(48) 1(1) −1(6.2) −1(25) 1(180) 1(1) −1(0) 1(12) 1(0.5) 380 337
11 1(48) 1(1) −1(6.2) −1(25) −1(120) −1(0) 1(1) −1(10) 1(0.5) 220 213
12 1(48) −1(0.5) 1(7.2) 1(28) 1(180) 1(1) −1(0) −1(10) 1(0.5) 360 361
13 1(24) −1(0.5) −1(6.2) −1(25) 1(180) −1(0) 1(1) −1(10) 1(0.5) 260 265
14 1(48) −1(0.5) 1(7.2) −1(25) 1(180) 1(1) 1(1) 1(12) −1(0.3) 230 241
15 1(24) 1(1) −1(6.2) 1(28) −1(120) 1(1) 1(1) 1(12) 1(0.5) 190 167
16 1(48) 1(1) 1(7.2) −1(25) −1(120) 1(1) 1(1) −1(10) 1(0.5) 160 207
17 −1(24) −1(0.5) −1(6.2) −1(25) −1(120) −1(0) −1(0) −1(10) −1(0.3) 320 303
18 1(48) −1(0.5) −1(6.2) 1(28) 1(180) −1(0) 1(1) 1(12) −1(0.3) 220 235
19 −1(24) −1(0.5) −1(6.2) 1(28) −1(120) 1(1) −1(0) 1(12) 1(0.5) 260 289
20 −1(24) 1(1) −1(6.2) 1(28) 1(180) 1(1) 1(1) −1(10) −1(0.3) 130 177
  1. X1: incubation time (h), X2: glucose % (w/v), X3: initial pH of production medium, X4: incubation temperature (°C), X5: agitation speed(rpm), X6: glycerol % (w/v)
  2. X7: starch % (w/v), X8: inoculum size % (v/v) and X9: yeast extract % (w/v). Values between in the parenthesis indicate real values of independent variables
  3. Exp experimental, Pred predicted
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