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Table 1 PBD showing levels of nine tested independent variables along with the response (SID EGY II activity)

From: Unusual non-fluorescent broad spectrum siderophore activity (SID EGYII) by Pseudomonas aeruginosa strain EGYII DSM 101801 and a new insight towards simple siderophore bioassay

Trial#

Independent variable

Dependent variable (Y) SID EGY II activity (SAU/mL)

X1

X2

X3

X4

X5

X6

X7

X8

X9

Exp

Pred

1

−1(24)

1(1)

1(7.2)

−1(25)

−1(120)

−1(0)

−1(0)

1(12)

−1(0.3)

280

327

2

1(48)

−1(0.5)

1(7.2)

1(28)

−1(120)

−1(0)

−1(0)

−1(10)

1(0.5)

330

353

3

1(48)

−1(0.5)

−1(6.2)

1(25)

−1(120)

1(1)

−1(0)

1(12)

−1(0.3)

290

277

4

1(48)

1(1)

1(7.2)

1(28)

−1(120)

−1(0)

1(1)

1(12)

−1(0.3)

250

209

5

−1(24)

−1(0.5)

1(7.2)

−1(25)

1(180)

−1(0)

1(1)

1(12)

1(0.5)

340

301

6

−1(24)

1(1)

1(7.2)

1(28)

1(180)

−1(0)

−1(0)

1(12)

1(0.5)

390

387

7

−1(24)

−1(0.5)

1(7.2)

1(28)

−1(120)

1(1)

1(1)

−1(10)

−1(0.3)

230

175

8

1(48)

1(1)

−1(6.2)

1(28)

1(180)

−1(0)

−1(0)

−1(10)

−1(.03)

340

327

9

−1(24)

1(1)

−1(7.2)

−1(25)

1(180)

1(1)

−1(0)

−1(10)

−1(0.3)

350

329

10

1(48)

1(1)

−1(6.2)

−1(25)

1(180)

1(1)

−1(0)

1(12)

1(0.5)

380

337

11

1(48)

1(1)

−1(6.2)

−1(25)

−1(120)

−1(0)

1(1)

−1(10)

1(0.5)

220

213

12

1(48)

−1(0.5)

1(7.2)

1(28)

1(180)

1(1)

−1(0)

−1(10)

1(0.5)

360

361

13

1(24)

−1(0.5)

−1(6.2)

−1(25)

1(180)

−1(0)

1(1)

−1(10)

1(0.5)

260

265

14

1(48)

−1(0.5)

1(7.2)

−1(25)

1(180)

1(1)

1(1)

1(12)

−1(0.3)

230

241

15

1(24)

1(1)

−1(6.2)

1(28)

−1(120)

1(1)

1(1)

1(12)

1(0.5)

190

167

16

1(48)

1(1)

1(7.2)

−1(25)

−1(120)

1(1)

1(1)

−1(10)

1(0.5)

160

207

17

−1(24)

−1(0.5)

−1(6.2)

−1(25)

−1(120)

−1(0)

−1(0)

−1(10)

−1(0.3)

320

303

18

1(48)

−1(0.5)

−1(6.2)

1(28)

1(180)

−1(0)

1(1)

1(12)

−1(0.3)

220

235

19

−1(24)

−1(0.5)

−1(6.2)

1(28)

−1(120)

1(1)

−1(0)

1(12)

1(0.5)

260

289

20

−1(24)

1(1)

−1(6.2)

1(28)

1(180)

1(1)

1(1)

−1(10)

−1(0.3)

130

177

  1. X1: incubation time (h), X2: glucose % (w/v), X3: initial pH of production medium, X4: incubation temperature (°C), X5: agitation speed(rpm), X6: glycerol % (w/v)
  2. X7: starch % (w/v), X8: inoculum size % (v/v) and X9: yeast extract % (w/v). Values between in the parenthesis indicate real values of independent variables
  3. Exp experimental, Pred predicted