Fig. 4

Toxicity of Bac7 (1–35) in E. coli. a The toxicity of pColdPrS₂-Bac7 (1–35) in E. coli. BL21(DE3) harboring either pColdP₂rS or pColdPrS₂-Bac7 (1–35). The cells were grown in the M9 medium. When OD₆₀₀ reached 0.2, PrS₂-Bac7 (1–35) was induced by 1 mM IPTG, and OD₆₀₀ was monitored every 30 min. The circles represent pColdPrS₂ without IPTG, the triangles pColdPrS₂-Bac7 (1–35) without IPTG, and the Xs pColdPrS₂ in the presence of IPTG and the squares pColdPrS₂-Bac7 (1–35). The time of the addition of IPTG is shown by an arrow. b The toxicity of purified Bac7 (1–35) in E. coli. The E. coli cells BL21(DE3) was grown in M9-glucose medium. When OD₆₀₀ reached 0.2, purified Bac7 (1–35) was added at the final concentration of 2 μM into the medium, and OD₆₀₀ was monitored every 30 min. The time of the addition of Bac7 (1–35) is shown by an arrow