Skip to main content

Table 3 Anti-AIV vector protection in chicken LMH cells as measured by log10 and fold-reductions in viral shedding titers compared to untreated controls

From: Inhibiting avian influenza virus shedding using a novel RNAi antiviral vector technology: proof of concept in an avian cell model

Sample n Adjusted meana (treated) 95 % CI (treated) Adjusted mean (PC) 95 % CI (PC) P valueb Log10 reductionc Fold reductiond
H8N4 virus MOI 0.01
 Anti-AIV/NP 9 1.1 (0.0, 2.6) 4.9 (3.7, 6.2) <0.001 3.8 6310
 Anti-AIV/PA 9 3.0 (2.1, 3.8) 5.5 (4.6, 6.4) <0.001 2.5 316
 Anti-AIV/cocktail 9 0.9 (0.0, 2.1) 4.9 (3.9, 6.0) <0.001 4.0 10,000
 Anti-AIV/scramble 10 2.8 (2.0, 3.5) 4.9 (4.1, 5.8) <0.001 2.1 126
H6N2 virus MOI 0.01
 Anti-AIV/NP 9 4.7 (3.1, 6.2) 6.3 (4.8, 8.0) 0.043 1.6 39.8
 Anti-AIV/PA 9 4.9 (3.6, 6.2) 6.3 (4.8, 7.9) 0.031 1.4 25.1
 Anti-AIV/cocktail 9 4.4 (3.0, 5.9) 6.3 (4.9, 7.8) 0.013 1.9 79.4
 Anti-AIV/scramble 9 5.5 (4.6, 6.3) 6.3 (5.3, 7.4) 0.053 0.8 6.3
  1. Mean viral titers were adjusted by day, using experimental day as a fixed effect
  2. aExpressed as mean log10 TCID50/mL adjusted by day
  3. bComparing adjusted mean log10 TCID50/mL from treated to untreated PC samples (p < 0.05)
  4. cLog10 reduction in mean infectious titer compared to untreated control
  5. dFold reduction in infectious titer (geometric mean) compared to untreated control