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Table 3 Anti-AIV vector protection in chicken LMH cells as measured by log10 and fold-reductions in viral shedding titers compared to untreated controls

From: Inhibiting avian influenza virus shedding using a novel RNAi antiviral vector technology: proof of concept in an avian cell model

Sample

n

Adjusted meana (treated)

95 % CI (treated)

Adjusted mean (PC)

95 % CI (PC)

P valueb

Log10 reductionc

Fold reductiond

H8N4 virus MOI 0.01

 Anti-AIV/NP

9

1.1

(0.0, 2.6)

4.9

(3.7, 6.2)

<0.001

3.8

6310

 Anti-AIV/PA

9

3.0

(2.1, 3.8)

5.5

(4.6, 6.4)

<0.001

2.5

316

 Anti-AIV/cocktail

9

0.9

(0.0, 2.1)

4.9

(3.9, 6.0)

<0.001

4.0

10,000

 Anti-AIV/scramble

10

2.8

(2.0, 3.5)

4.9

(4.1, 5.8)

<0.001

2.1

126

H6N2 virus MOI 0.01

 Anti-AIV/NP

9

4.7

(3.1, 6.2)

6.3

(4.8, 8.0)

0.043

1.6

39.8

 Anti-AIV/PA

9

4.9

(3.6, 6.2)

6.3

(4.8, 7.9)

0.031

1.4

25.1

 Anti-AIV/cocktail

9

4.4

(3.0, 5.9)

6.3

(4.9, 7.8)

0.013

1.9

79.4

 Anti-AIV/scramble

9

5.5

(4.6, 6.3)

6.3

(5.3, 7.4)

0.053

0.8

6.3

  1. Mean viral titers were adjusted by day, using experimental day as a fixed effect
  2. aExpressed as mean log10 TCID50/mL adjusted by day
  3. bComparing adjusted mean log10 TCID50/mL from treated to untreated PC samples (p < 0.05)
  4. cLog10 reduction in mean infectious titer compared to untreated control
  5. dFold reduction in infectious titer (geometric mean) compared to untreated control