Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor

AMB Express

Table 1 Process parameter of different media setups of the P. putida—rhamnolipid system and B. subtilis—surfactin system

	P. putida—rhamnolipid in Wilms		P. putida—rhamnolipid in ModR		B. subtilis—surfactin
Overall Y_P/X (g/g)	0.06	±0.01	0.10	±0.01	0.15	±0.07
Overall Y_X/S (g/g)	0.16	±0.02	0.26	±0.02	0.24	±0.06
Max. q_Product [mg/(g∙h)]	5.90	±0.29	24.71	±0.69	45.00	±26.93
Integral q_Product [mg/(g∙h)]	1.89	±0.44	5.57	±0.39	4.37	±1.98
Max. vol. q_Product [mg/(L∙h)]	8.70	±1.16	38.09	±2.12	46.25	±35.68
Overall product recovery (%)	83.39	±1.51	97.38	±0.33	85.35	±13.19
Max. product enrichment	128.54	±36.54	197.98	±13.42	80.68	±39.73
Mean bact. enrichment	0.25	±0.07	0.86	±0.19	0.79	±0.45
Max. product conc. in foam (g/L)	1.04	±0.04	2.15	±0.04	2.35	±0.76
Product recovered in foam (g)	0.30	±0.02	0.83	±0.03	0.53	±0.35

The mean of different process parameters of all B. subtilis—surfactin systems is depicted (Willenbacher et al. 2014). In P. putida—rhamnolipid systems Y_P/X, Y_X/S, max. q_Product, integral q_Product and max. vol q_Product were calculated using logistic fit data whereas all other parameter were calculated using measured data