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Table 2 Experimental design Box-Behnken for three independent variables and experimental data with 13 trials

From: Combining glucose and sodium acetate improves the growth of Neochloris oleoabundans under mixotrophic conditions

Run Independent variables Dependent variables
Glucose Sodium acetate g L−1 BBM (%) OD670 Dry biomass g L−1
    10 day 15 day 10 day 15 day
1 −1 (0.0) −1 (0.0) 0 (75) 0.170 0.100 0.080 0.100
2 1 (10.0) −1 (0.0) 0 (75) 0.750 0.826 0.725 0.485
3 −1 (0.0) 1 (10.0) 0 (75) 0.203 0.215 0.264 0.189
4 1 (10.0) 1 (10.0) 0 (75) 1.422 1.619 0.921 1.233
5 −1 (0.0) 0 (5.0) −1 (50) 0.305 0.260 0.150 0.180
6 1 (10.0) 0 (10.0) 1 (50) 1.549 1.555 0.962 1.029
7 −1 (0.0) 0 (5.0) 1 (100) 0.340 0.304 0.240 0.200
8 1 (10.0) 0 (5.0) 1 (100) 1.729 1.452 1.195 1.048
9 0 (5.0) −1 (0.0) −1 (50) 0.726 0.699 0.436 0.456
10 0 (5.0) 1 (10.0) −1 (50) 1.423 1.504 0.911 0.989
11 0 (5.0) −1 (0.0) 1 (100) 1.133 1.047 0.762 0.636
12 0 (5.0) 1 (10.0) 1 (100) 1.807 1.378 1.529 0.971
13 0 (5.0) 0 (5.0) 0 (75) 1.677 1.649 1.223 1.059
  1. The optical density at 670 nm (OD670) and dry biomass determinations N. Oleoabundans were performed after 10 and 15 days of cultivation