AMB Express

Table 2 Experimental design Box-Behnken for three independent variables and experimental data with 13 trials

From: Combining glucose and sodium acetate improves the growth of Neochloris oleoabundans under mixotrophic conditions

Run	Independent variables			Dependent variables
	Glucose	Sodium acetate g L⁻¹	BBM (%)	OD₆₇₀		Dry biomass g L⁻¹
				10 day	15 day	10 day	15 day
1	−1 (0.0)	−1 (0.0)	0 (75)	0.170	0.100	0.080	0.100
2	1 (10.0)	−1 (0.0)	0 (75)	0.750	0.826	0.725	0.485
3	−1 (0.0)	1 (10.0)	0 (75)	0.203	0.215	0.264	0.189
4	1 (10.0)	1 (10.0)	0 (75)	1.422	1.619	0.921	1.233
5	−1 (0.0)	0 (5.0)	−1 (50)	0.305	0.260	0.150	0.180
6	1 (10.0)	0 (10.0)	1 (50)	1.549	1.555	0.962	1.029
7	−1 (0.0)	0 (5.0)	1 (100)	0.340	0.304	0.240	0.200
8	1 (10.0)	0 (5.0)	1 (100)	1.729	1.452	1.195	1.048
9	0 (5.0)	−1 (0.0)	−1 (50)	0.726	0.699	0.436	0.456
10	0 (5.0)	1 (10.0)	−1 (50)	1.423	1.504	0.911	0.989
11	0 (5.0)	−1 (0.0)	1 (100)	1.133	1.047	0.762	0.636
12	0 (5.0)	1 (10.0)	1 (100)	1.807	1.378	1.529	0.971
13	0 (5.0)	0 (5.0)	0 (75)	1.677	1.649	1.223	1.059

The optical density at 670 nm (OD₆₇₀) and dry biomass determinations N. Oleoabundans were performed after 10 and 15 days of cultivation

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