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Table 2 Experimental design Box-Behnken for three independent variables and experimental data with 13 trials

From: Combining glucose and sodium acetate improves the growth of Neochloris oleoabundans under mixotrophic conditions

Run

Independent variables

Dependent variables

Glucose

Sodium acetate g L−1

BBM (%)

OD670

Dry biomass g L−1

   

10 day

15 day

10 day

15 day

1

−1 (0.0)

−1 (0.0)

0 (75)

0.170

0.100

0.080

0.100

2

1 (10.0)

−1 (0.0)

0 (75)

0.750

0.826

0.725

0.485

3

−1 (0.0)

1 (10.0)

0 (75)

0.203

0.215

0.264

0.189

4

1 (10.0)

1 (10.0)

0 (75)

1.422

1.619

0.921

1.233

5

−1 (0.0)

0 (5.0)

−1 (50)

0.305

0.260

0.150

0.180

6

1 (10.0)

0 (10.0)

1 (50)

1.549

1.555

0.962

1.029

7

−1 (0.0)

0 (5.0)

1 (100)

0.340

0.304

0.240

0.200

8

1 (10.0)

0 (5.0)

1 (100)

1.729

1.452

1.195

1.048

9

0 (5.0)

−1 (0.0)

−1 (50)

0.726

0.699

0.436

0.456

10

0 (5.0)

1 (10.0)

−1 (50)

1.423

1.504

0.911

0.989

11

0 (5.0)

−1 (0.0)

1 (100)

1.133

1.047

0.762

0.636

12

0 (5.0)

1 (10.0)

1 (100)

1.807

1.378

1.529

0.971

13

0 (5.0)

0 (5.0)

0 (75)

1.677

1.649

1.223

1.059

  1. The optical density at 670 nm (OD670) and dry biomass determinations N. Oleoabundans were performed after 10 and 15 days of cultivation