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AMB Express
Fig. 7 | AMB Express

Fig. 7

From: The roles of the zinc finger transcription factors XlnR, ClrA and ClrB in the breakdown of lignocellulose by Aspergillus niger

Fig. 7

Reactive oxygen species (ROS) accumulation and cell permeability using the dyes H2DCF-DA (a) and phloxine B (b). Phloxine B stains the cytosolic material due to a more permeable cell in the ΔxlnR and ΔclrB strains. H2DCF-DA emits a green fluorescence when it is oxidised by ROS in the cell. The fluorescence intensity was measured using the ImageJ software. The results shown represent 50 measurements of three independent biological samples and the error bars indicate the standard error of the mean. * represents the conditions for which the t-test result had a p-value < 0.05 which means that the difference observed is significant when compared to the WT. c Expression of the alternative oxidase gene (aox1) measured by qRT-PCR in the WT and the ΔxlnR and ΔclrB strains. The results shown represent triplicates of three independent biological samples and the error bars indicate the standard error of the mean

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