Disruption of PHO13 improves ethanol production via the xylose isomerase pathway

Bamba, Takahiro; Hasunuma, Tomohisa; Kondo, Akihiko

doi:10.1186/s13568-015-0175-7

AMB Express

Table 3 Comparison of xylose assimilation by XI-harboring recombinant strains

From: Disruption of PHO13 improves ethanol production via the xylose isomerase pathway

Strain	Description	Adaptation process	Fermentation conditions	Medium	Specific xylose consumption rate (g/g-cell/h)	Specific ethanol productivity (g/g-cell/h)	Ethanol yield (g/g-xylose)	References
YΔG/XK/XI	Orpinimyces xylA, XKS1, ΔGRE3	–	Oxygen-limited batch fermentation in 100-mL bottle	C	0.23	0.08	0.42	This study
YΔGP/XK/XI	Orpinimyces xylA, XKS1, ΔGRE3, ΔPHO13	–	Oxygen-limited batch fermentation in 100-mL bottle	C	0.31	0.11	0.45	This study
SXA-R2P-E	Piromyces xylA variant, Scheffersomyces stipitis tal1, XKS1, ΔGRE3, ΔPHO13, Evolved	Xylose adaptation	Oxygen-limited batch fermentation in 50-mL vial	S	0.14	0.05	NR	Lee et al. (2014)
SXA-R2P-E	Piromyces xylA variant, Scheffersomyces stipitis tal1, XKS1, ΔGRE3, ΔPHO13, Evolved	Xylose adaptation	Anaerobic batch fermentation in bioreactor	S + a. g.	0.98	0.44	0.45	Lee et al. (2014)
H131-A3-AL^CS	Piromyces xylA, S. stipitis XYL3, S. stipitis TAL1, TKL1, RPE1, RKI1, ARG4, LEU2, Evolved	Xylose adaptation	Anaerobic batch fermentation in bioreactor	2 × S + a. g.	1.87	0.77	0.41	Zhou et al. (2012)
ADAP8	Orpinimyces xylA, XKS1, SUT1, Evolved	Xylose adaptation	Oxygen-limited batch fermentation in 100-mL bottle	C	NR	0.038	0.48	Madhavan et al. (2009b)
TMB3066	Piromyces xylA, XKS1, TAL1, TKL1, RKI1, RPE1, ΔGRE3	–	Anaerobic batch fermentation in 25-mL serum flask	S + a. g.	0.05	0.02	0.43	Karhumaa et al. (2007)
RWB 217	Piromyces xylA, XKS1, TAL1, TKL1, RKI1, RPE1, ΔGRE3	–	Anaerobic batch fermentation in bioreactor	S + a. g.	1.06	NR	0.43	Kuyper et al. (2005a)

NR not reported, C complex medium (10 g/L yeast extract, 20 g/L peptone), S synthetic medium (6.7 g/L yeast nitrogen base, appropriate nucleotides and amino acids), a. g. anaerobic growth factors (0.01 g/L ergosterol, 0.4 g/L Tween 80)

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