Fig. 2From: Molecular breeding of lignin-degrading brown-rot fungus Gloeophyllum trabeum by homologous expression of laccase geneDirect PCR analysis of genomic DNA recovered from individual transformants. Supernatants of homogenized mycelium of the transformants were used in PCR amplification with primer pair hptF1-hptR1 (a) or gpdF12-lccR12 (b). “M” indicates a 100 bp ladder size marker. “NC” indicates negative control (reactions with no PCR template). “PC” indicates positive control using pGL as PCR template. The PCR products were electrophoresed in 2 % agar and stained with SYBR Green (Takara)Back to article page