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Table 2 Experimental design and conversion yields of mango, neem and shea oils

From: ESolvent-free, enzyme-catalyzed biodiesel production from mango, neem, and shea oils via response surface methodology

S/no

Experimental matrix

Responses

Coded values

Real values

Conversion yield (%)

x1

x2

x3

x4

X 1

X 2

X 3

X4

Neem

Mango

Shea

1

1

0

0

0

10.00

45.00

10.00

24.00

91.68

92.27

98.77

1

1

0

0

0

10.00

45.00

10.00

24.00

92.71

91.11

99.70

2

−1

0

0

0

3.00

45.00

10.00

24.00

90.51

56.24

58.23

2

−1

0

0

0

3.00

45.00

10.00

24.00

90.77

61.69

60.72

3

0.5

0.866

0

0

8.25

60.00

10.00

24.00

71.74

63.87

99.03

3

0.5

0.866

0

0

8.25

60.00

10.00

24.00

68.98

68.45

99.31

4

−0.5

−0.866

0

0

4.75

30.00

10.00

24.00

94.76

82.69

86.03

4

−0.5

−0.866

0

0

4.75

30.00

10.00

24.00

96.24

85.15

84.15

5

0.5

−0.866

0

0

8.25

30.00

10.00

24.00

93.64

91.38

95.48

5

0.5

−0.866

0

0

8.25

30.00

10.00

24.00

94.34

89.37

94.98

6

−0.5

0.866

0

0

4.75

60.00

10.00

24.00

82.41

48.39

72.86

6

−0.5

0.866

0

0

4.75

60.00

10.00

24.00

86.94

49.38

73.74

7

0.5

0.289

0.816

0

8.25

50.01

15.00

24.00

92.54

91.01

82.70

7

0.5

0.289

0.816

0

8.25

50.01

15.00

24.00

92.53

89.77

85.19

8

−0.5

−0.289

−0.816

0

4.75

39.99

5.00

24.00

89.29

68.70

94.55

8

−0.5

−0.289

−0.816

0

4.75

39.99

5.00

24.00

85.71

70.11

94.70

9

0.5

−0.289

−0.816

0

8.25

39.99

5.00

24.00

93.33

90.82

96.61

9

0.5

−0.289

−0.816

0

8.25

39.99

5.00

24.00

92.52

90.88

97.11

10

0

0.577

−0.816

0

6.50

54.99

5.00

24.00

89.82

77.04

97.07

10

0

0.577

−0.816

0

6.50

54.99

5.00

24.00

88.52

73.64

97.70

11

−0.5

0.289

0.816

0

4.75

50.01

15.00

24.00

97.17

84.99

60.62

11

−0.5

0.289

0.816

0

4.75

50.01

15.00

24.00

93.58

75.32

59.74

12

0

−0.577

0.816

0

6.50

35.01

15.00

24.00

98.68

93.14

81.77

12

0

−0.577

0.816

0

6.50

35.01

15.00

24.00

97.65

92.52

78.24

13

0.5

0.289

0.204

0.791

8.25

50.01

11.25

36.00

97.88

96.30

100.00

13

0.5

0.289

0.204

0.791

8.25

50.01

11.25

36.00

98.90

98.36

99.89

14

−0.5

−0.289

−0.204

−0.791

4.75

39.99

8.75

12.00

93.44

45.32

66.50

14

−0.5

−0.289

−0.204

−0.791

4.75

39.99

8.75

12.00

95.30

47.85

62.75

15

0.5

−0.289

−0.204

−0.791

8.25

39.99

8.75

12.00

95.24

58.58

92.99

15

0.5

−0.289

−0.204

−0.791

8.25

39.99

8.75

12.00

95.46

57.11

97.78

16

0

0.577

−0.204

−0.791

6.50

54.99

8.75

12.00

95.58

71.97

99.78

16

0

0.577

−0.204

−0.791

6.50

54.99

8.75

12.00

95.63

71.15

95.09

18

−0.5

0.289

0.204

0.791

4.75

50.01

11.25

36.00

99.58

88.42

99.65

18

−0.5

0.289

0.204

0.791

4.75

50.01

11.25

36.00

98.52

87.77

99.85

19

0

−0.577

0.204

0.791

6.50

35.01

11.25

36.00

99.79

96.24

99.61

19

0

−0.577

0.204

0.791

6.50

35.01

11.25

36.00

99.80

96.74

99.54

20

0

0

−0.612

0.791

6.50

45.00

6.25

36.00

98.39

97.14

99.54

20

0

0

−0.612

0.791

6.50

45.00

6.25

36.00

95.54

96.33

99.54

21

0

0

0

0

6.50

45.00

10.00

24.00

90.70

97.34

96.62

21

0

0

0

0

6.50

45.00

10.00

24.00

91.43

93.58

98.22

R2

        

0.808

0.882

0.949

AED (%)\

        

2.58

5.95

2.85

  1. X 1 enzyme concentration, X 2 reaction temperature, X 3 quantity of added water and X 2 reaction time