Accessing inoculation methods of maize and wheat with Azospirillum brasilense

AMB Express

Table 6 Shoot dry weight (SDW) and N content (NC) in maize plants from field experiments performed in three different regions of Brazil (Cachoeira Dourada, hybrid 2B707 HX, Luis Eduardo Magalhães, hybrid 2B707 HX, and Ponta Grossa, hybrid P4285 H), in response to different doses of N fertilizer, doses of inoculant and methods of inoculation with Azospirillum brasilens

Treatment	Cachoeira Dourada		Luis E. Magalhães		Ponta Grossa
Treatment	SDW (g pl⁻¹)	NC (mg g⁻¹)	SDW (g pl⁻¹)	NC (mg g⁻¹)	SDW (g pl⁻¹)	NC (mg g⁻¹)
T1: C	29.45 c	23.08 a	68.82^ns	30.08 b	49.08^ns	18.91 d
T2: C + 100 % N^a	36.01 abc	22.19 a	66.30	30.05 b	53.50	23.69 ab
T3: C + 75 % N	33.01 ab	21.16 ab	65.78	30.97 ab	56.09	23.74 ab
T4: SI + 1 dose^b + 100 % N	36.68 abc	20.93 ab	71.34	31.60 ab	54.12	24.67 a
T5: SI + 1 dose + 75 % N	41.13 a	21.60 ab	71.26	33.43 ab	58.33	23.86 ab
T6: IPF + 2 doses + 75 % N	34.41 abc	20.31 abc	70.50	32.79 ab	53.95	21.11 c
T7: IPF + 4 doses + 75 % N	34.23 abc	19.18 bc	65.45	34.10 ab	54.14	22.15 bc
T8: ILS + 2 doses + 75 % N	34.26 abc	19.07 bc	69.75	35.31 a	49.48	22.71 bc
T9: ILS + 4 doses + 75 % N	36.34 abc	18.68 bc	77.63	33.88 ab	54.97	20.94 c
T10: ISS + 2 doses + 75 % N	39.47 ab	17.69 c	71.94	30.58 b	50.60	20.82 c
T11: ISS + 4 doses + 75 % N	32.18 bc	20.76 ab	75.41	31.36 ab	47.73	20.96 c
p value	0.0438	0.0028	0.5303	0.0764	0.4597	<0.0001
CV (%)	34.64	10.82	13.97	10.07	15.15	6.77

Plants harvested at 30, 56 and 61 days after emergence, in Cachoeira Dourada, Luis Eduardo Magalhães and Ponta Grossa, respectively
Means (six replicates) followed by the same letter on the column are not significantly different from one another according to Duncan’s test (p ≤ 0.05). ns not significant
C non-inoculated control, SI standard seed inoculation at sowing, IPF inoculation at planting in the furrow, ILS inoculation by leaf spray at the V2.5 stage, ISS inoculation by soil spray at the V2.5 stage
^aN, 75 % (24 kg ha⁻¹ at sowing + 67.5 kg ha⁻¹ as side dress); 100 % (24 kg ha⁻¹ at sowing + 90 kg ha⁻¹ as side dress)
^bDose, 1 dose (1.0 × 10⁵ cells seeds⁻¹ or 1.0 × 10⁵ cells plant⁻¹)