Table 4 Shoot dry weight (SDW), root dry weight (RDW), N content (NC), total N accumulated in the shoots (TNS), chlorophyll content (CC), root volume (RV), culm diameter (CD), plant height (PH), and MPN (most probable number) of diazotrophic bacteria on leaves, roots and rhizosphere in a greenhouse experiment performed with hybrid maize BRS 3010 in response to different doses of inoculant, levels of N fertilization, and methods of inoculation
From: Accessing inoculation methods of maize and wheat with Azospirillum brasilense
Treatment | SDW (g pl−1) | RDW (g pl−1) | NC (mg g−1) | TNS (mg pl−1) | CC (µg cm−2) | Biometric measurements | Bacterial MPN | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
RV (mL pl−1) | CD (mm) | PH (cm) | Leaves (n° cells g−1) | Roots (n° cells g−1) | Rhizosphere soil (n° cells g−1) | |||||||
T1: C + 75 % Na | 6.67 c* | 3.77 bc | 14.74 abc | 98.33 cde | 12.99ns | 19.90 abc | 9.47ns | 66.60 bcd | 4.57 × 106 cde | 1.73 × 108 e | 1.55 × 106 b | |
T2: C + 100 % N | 8.01 ab | 3.96 bc | 14.41 abcd | 115.60 abc | 13.71 | 20.30 abc | 10.12 | 73.12 ab | 2.92 × 106 de | 4.75 × 108 bcde | 5.52 × 105 b | |
T3: SI + 1 doseb + 75 % N | 7.43 abc | 3.92 bc | 11.81 de | 87.03 e* | 12.93 | 18.90 bc | 9.97 | 64.40 bcd | 2.47 × 106 de | 6.24 × 108 abcde | 6.12 × 105 b | |
T4: SI +1 dose + 100 % N | 7.56 abc | 3.66 c | 15.17 ab | 113.99 abc | 14.76 | 19.40 bc | 9.93 | 62.10 d | 1.14 × 107 bcde | 6.24 × 108 abcde | 2.12 × 106 b | |
T5: SI + 2.5 doses + 75 % N | 7.71 abc | 3.95 bc | 14.96 ab | 115.93 abc | 12.33 | 20.40 abc | 10.08 | 64.60 bcd | 4.31 × 106 cde | 6.86 × 108 abcde | 5.10 × 105 b | |
T6: SI + 2.5 doses + 100 % N | 8.02 ab | 4.47 ab | 14.74 abc | 117.91 abc | 12.99 | 23.30 a | 10.22 | 66.50 bcd | 1.16 × 108 b | 2.83 × 108 cde | 5.10 × 105 b | |
T7: IPF + 1 dose + 75 % N | 7.29 bc | 4.11 bc | 15.72 ab | 114.39 abc | 12.29 | 18.70 bc | 10.08 | 65.50 bcd | 1.22 × 108 b* | 5.03 × 108 abcde | 3.02 × 106 b | |
T8: IPF + 1 dose + 100 % N | 7.59 abc | 3.93 bc | 15.96 ab | 123.21 a | 13.72 | 19.10 bc | 10.58 | 70.75 abcd | 2.70 × 107 bcd | 4.58 × 108 abcde | 2.52 × 107 a* | |
T9: IPF + 2.5 doses + 75 % N | 8.16 ab | 4.07 bc | 11.33 e* | 91.93 de | 12.18 | 19.60 bc | 10.22 | 65.70 bcd | 2.18 × 107 bcd | 1.25 × 109 a* | 1.52 × 106 b | |
T10: IPF + 2.5 doses + 100 % N | 7.57 abc | 3.88 bc | 14.66 abcd | 109.79 abcd | 13.30 | 18.40 bc | 10.07 | 69.80 abcd | 2.40 × 109 a* | 3.28 × 108 bcde | 8.48 × 107 b | |
T11: ILS + 1 dose + 75 % N | 7.30 abc | 3.92 bc | 13.62 abcde | 99.49 cde | 12.28 | 20.30 abc | 10.25 | 68.80 abcd | 2.05 × 109 a* | 2.37 × 108 de | 1.03 × 106 b | |
T12: ILS + 1 dose + 100 % N | 7.39 abc | 4.17 bc | 13.48 abcde | 98.64 cde | 13.38 | 20.20 abc | 10.58 | 62.87 cd | 7.38 × 107 bc | 1.02 × 10 abc | 4.65 × 106 ab | |
T13: ILS + 2.5 doses + 75 %N | 7.22 bc | 4.41 abc | 13.47 abcde | 96.92 cde | 12.38 | 21.30 ab | 10.07 | 70.20 abcd | 7.50 × 106 bcde | 9.18 × 108 abcd | 1.54 × 106 b | |
T14: ILS + 2.5 doses + 100 % N | 8.13 ab | 5.00 a* | 13.07 bcde | 106.06 abcde | 12.63 | 21.00 abc | 10.30 | 72.80 cd | 1.12 × 106 de | 7.33 × 108 abcde | 3.04 × 106 b | |
T15: ISS + 1 dose + 75 % N | 7.52 abc | 3.60 c | 13.14 abcde | 98.30 cde | 13.29 | 18.10 bc | 9.84 | 68.70abcd | 4.07 × 106 cde | 4.84 × 108 cde | 1.34 × 106 b | |
T16: ISS + 1 dose + 100 % N | 7.92 abc | 4.38 abc | 16.05 a | 126.731 a | 13.97 | 20.50 abc | 10.07 | 75.70a | 1.10 × 106 de | 9.94 × 108 abc | 1.40 × 106 b | |
T17: ISS + 2.5 doses + 75 % N | 8.50 a | 4.47 ab | 11.95 cde | 101.15 bcde | 11.80 | 21.30 ab | 10.43 | 73.10 ab | 1.11 × 106 de | 3.66 × 108 cde | 1.50 × 106 b | |
T18: ISS + 2.5 doses + 100 % N | 7.72 abc | 3.88 bc | 15.71 ab | 121.13 ab | 13.91 | 17.70 c | 10.21 | 71.40 abc | 6.25 × 105 e | 1.14 × 109 ab | 1.65 × 106 b | |
p value | 0.0058 | 0.015 | <0.0001 | <0.0001 | 0.5525 | 0.00492 | 0.6696 | 0.0127 | <0.0001 | 0.0192 | 0.0002 | |
CV (%) | 8.08 | 12.81 | 10.57 | 10.06 | 13.92 | 12.02 | 6.38 | 10.6 | 11.2 | 6.65 | 9.24 | |