Fig. 5
From: Coexpression of cellulases in Pichia pastoris as a self-processing protein fusion
Purification of individual enzymes after protein fusion cleavage. The supernatant from clone FUSAOX was applied on a Q-Sepharose and proteins were eluted on a NaCl gradient. Sample fractions were analyzed on 12 % SDS-PAGE. CBHII was eluted on fractions 10–13 and EGII on fractions 15 and 16. The rectangles indicate individual enzymes. Fractions 12 and 16 were tested for cellulase activity. Only relevant bands of the unstained protein molecular weight marker (Thermo Scientific) are indicated by arrows at the right side of the panel