Table 1 Designs of primers used in this study
From: Synergistic properties of cellulases from Clostridium cellulovorans in the presence of cellobiose
Primer | Sequence | Restriction site | Prasmid |
|---|---|---|---|
engH-TF-F | GTTCTCGAGTTATCAGGAATCTTGGGTGCAACTTC | XhoI | pCold-TF-engH |
engH-TF R | TTAGGATCCCTGATAAAAGTAG | BamHI | pCold-TF-engH |
sacI-engK | TTGAGCTCATGCGTAGTAAAAAATTAATAGCTTG | SacI | pCold-I-engK |
engK-xhoI | CCCCTCGAGTTAAGAAAGAAGTTTCTTCT | XhoI | pCold-I-engK |
sacI-exgS | GGGAGCTCATGAGAAAAAGATTAAATAAGATCGTTG | SacI | pCold-I-exgS |
exgS-xhoI | CCCCTCGAGTTAAGCAAGAAGTGCTTTCT | XhoI | pCold-I-exgS |