The binding of cellulase variants to dislocations: a semi-quantitative analysis based on CLSM (confocal laser scanning microscopy) images

AMB Express

Table 2 t-test to determine the significance of the difference between the mean of R values of antibody-CBM_EGV and non-CBM EGVs

	Antibody-CBM_EGV	EGV without CBM	EGV inactive without CBM	Antibody-CBM_EGV vs EGV without CBM	Antibody-CBM_EGV vs EGV inactive without CBM
Mean R	2.08	1.22	1.41
Number of samples	28	28	22
Total samples				28 + 28 = 56	28 + 22 = 50
2-Tailed probability points of t-distribution at 5 % level				2.01	2.01
Standard error of measurement*				0.26	0.28
Mean difference				2.08 − 1.22 = 0.86	2.08 − 1.41 = 0.67
95 % confidence interval for the difference between population means				0.86 ± 2.01 × 0.26 or 0.43–1.26	0.67 ± 2.01 × 0.28 or 0.19–1.15
				As the 95 % confidence interval does not include 0, the difference between the two means is real

*Calculated according to the formula SEM = (s² × (1/n1 + 1/n2))^0.5, where s is the combined variance of the two population involved = (SS1 + SS2)/(n1 + n2 − 2), while SS1 and SS2 are the sum of squares of the respective population and n1 and n2 are the number of samples of the respective population