The binding of cellulase variants to dislocations: a semi-quantitative analysis based on CLSM (confocal laser scanning microscopy) images

AMB Express

Table 1 Enzymes/proteins used in this study

Enzymes	MW (kDa)	Number of constituent amino acids	Number of CLSM images
Enzymes	MW (kDa)	Number of constituent amino acids	Non washed samples	Washed samples
Humicola insolens EGV	43	284	12	8
Humicola insolens EGV, non CBM	31.2	213	21	11
Humicola insolens EGV, non CBM, inactive^a	31.2	213	15	9
Antibody-CBM ^b_EGV	31.6	216	18	12
Trichoderma reesei CBHI	68	497	8	6
Trichoderma reesei CBHI, non CBM	50	436	11	3
Trichoderma reesei CBHII	58	365	10	4
Trichoderma reesei EGII	48	398	11	4

The size of the enzymes and the number of CLSM images obtained for each enzyme are shown
^aIs a H. insolens EGV Active Domain with a single mutation at its active site
^bIs a chimeric protein having similar size with the Active Domain of H. insolens EGV and constructed by combining CBM from H. insolens EGV and an antibody molecule