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Fig. 6 | AMB Express

Fig. 6

From: The binding of cellulase variants to dislocations: a semi-quantitative analysis based on CLSM (confocal laser scanning microscopy) images

Fig. 6

Trichoderma reesei CBHI (non CBM) binding to a fiber. a PLM image showing dislocations; ROIs are shown for bulk solution (red square), normal cell wall (green square), dislocations (blue square), and lumen (orange square). b CLSM images showing binding at 4 different levels across z-axis of the microscope as shown in c. c Binding profile across z-axis within ROIs shown in a (same colour-code). SOI (Slices of Interest; 4 slices) between 6.5 and 11.5 μm (boxed region) were selected for R calculation. Fluorescence intensity level in arbitrary unit. T. reesei CBHI (non CBM) does not show increased binding to the dislocations. The size of the images is 119.16 × 59.58 μm2

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