Fig. 5

Trichoderma reesei CBHI binding to a fiber. a PLM image showing dislocations; ROIs are shown for bulk solution (red square), normal cell wall (green square), dislocations (blue square), and lumen (orange square). b CLSM images showing binding at 4 different levels across z-axis of the microscope as shown in c. c Binding profile across z-axis within ROIs shown in a (same colour-code). SOIs (Slices of Interest; 5 slices) between 7.8 and 14 μm (boxed region) were selected for R calculation. Fluorescence intensity level in arbitrary unit. T. reesei CBHI does not show increased binding to dislocations. The size of the images is 119.16 × 59.58 μm²