Fig. 4

Humicola insolens EGV (non CBM) binding to a fiber. a PLM image showing dislocations; ROIs are shown for bulk solution (red square), normal cell wall (green square), dislocations (blue square), and lumen (orange square). b CLSM images showing binding at 4 different levels across z-axis of the microscope as shown in c. c Binding profile across z-axis within ROIs shown in a (same colour-code). SOIs (Slices of Interest; 4 slices) between 6.2–9.3 μm (boxed region) were selected for R calculation. Fluorescence intensity level in arbitrary unit. H. insolens (non CBM) does not show increased binding to dislocations. The size of the images is 119.16 × 59.58 μm²