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AMB Express

Table 5 Stability of purified GlcDH-BS and GlcDH-BA in various organic solvents

From: Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration

Solvents

logPo/w

% Relative activitya

GluDH-BS

GluDH-BA

10 % v/v

50 % v/v

10 % v/v

50 % v/v

DMSO

−1.4

87 ± 3

98 ± 3

100 ± 0

99 ± 1

Acetone

−0.2

80 ± 2

1 ± 1

99 ± 1

1 ± 1

Ethanol

−0.2

86 ± 3

1 ± 1

100 ± 1

19 ± 16

n-Butanol

0.9

0 ± 0.5

0 ± 0

2 ± 0

1 ± 1

n-Hexane

3.8

89 ± 0

99 ± 1

100 ± 0

100 ± 0

1-Hexanol

1.8

56 ± 10

n.a.

94 ± 0

n.a.

2-Hexanol

1.8

68 ± 4

69 ± 1

95 ± 1

93 ± 2

  1. The relative activity was calculated based on an activity of a control without organic solvent (water was added instead)
  2. n.a. data not available
  3. aThe activity was determined using CPB (pH 6) at 37 °C after an exposure with a specified organic solvent at 30 °C for 1 h
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