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Table 5 Stability of purified GlcDH-BS and GlcDH-BA in various organic solvents

From: Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration

Solvents logPo/w % Relative activitya
GluDH-BS GluDH-BA
10 % v/v 50 % v/v 10 % v/v 50 % v/v
DMSO −1.4 87 ± 3 98 ± 3 100 ± 0 99 ± 1
Acetone −0.2 80 ± 2 1 ± 1 99 ± 1 1 ± 1
Ethanol −0.2 86 ± 3 1 ± 1 100 ± 1 19 ± 16
n-Butanol 0.9 0 ± 0.5 0 ± 0 2 ± 0 1 ± 1
n-Hexane 3.8 89 ± 0 99 ± 1 100 ± 0 100 ± 0
1-Hexanol 1.8 56 ± 10 n.a. 94 ± 0 n.a.
2-Hexanol 1.8 68 ± 4 69 ± 1 95 ± 1 93 ± 2
  1. The relative activity was calculated based on an activity of a control without organic solvent (water was added instead)
  2. n.a. data not available
  3. aThe activity was determined using CPB (pH 6) at 37 °C after an exposure with a specified organic solvent at 30 °C for 1 h