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Table 3 Kinetic constants of purified GluDH from B. amyloliquefaciens SB5 comparing to those reported in the literature

From: Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration

Microorganism K M value (mM) Turnover number (1/s)a Specific activity (U/mg-protein) Optimal pH References
NAD+ NADP+ β-D-glucosea
Bacillus subtilis 0.23 0.26 42.9 n.a.c 26 9 Fujita et al. (1977)
Gluconobacter suboxydans n.d.b 0.01 5 n.a.c 140 6 Adachi et al. (1980)
Sulfolobus solfataricus 1.2 0.03 0.44 48 437 8 Giardina et al. (1986)
Bacillus megaterium 0.37 0.03 11 260 n.a.c 6.5 Yamamoto et al. (1990)
Lysinibacillus sphaericus G10 0.09 0.07 5.1 93 205 9.5 Ding et al. (2011)
Bacillus amyloliquefaciens SB5 0.25 ± 0.03 0.05 ± 0.004 5.5 ± 0.28 70 ± 0.3 123 ± 0.5 10 This study
  1. All kinetic parameters of GluDH-BA are presented as Avg ± SD
  2. aWith NADP+
  3. bNo activity with NAD+
  4. cData not available