Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration

AMB Express

Table 3 Kinetic constants of purified GluDH from B. amyloliquefaciens SB5 comparing to those reported in the literature

Microorganism	K _M value (mM)			Turnover number (1/s)^a	Specific activity (U/mg-protein)	Optimal pH	References
Microorganism	NAD⁺	NADP⁺	β-D-glucose^a	Turnover number (1/s)^a	Specific activity (U/mg-protein)	Optimal pH	References
Bacillus subtilis	0.23	0.26	42.9	n.a.^c	26	9	Fujita et al. (1977)
Gluconobacter suboxydans	n.d.^b	0.01	5	n.a.^c	140	6	Adachi et al. (1980)
Sulfolobus solfataricus	1.2	0.03	0.44	48	437	8	Giardina et al. (1986)
Bacillus megaterium	0.37	0.03	11	260	n.a.^c	6.5	Yamamoto et al. (1990)
Lysinibacillus sphaericus G10	0.09	0.07	5.1	93	205	9.5	Ding et al. (2011)
Bacillus amyloliquefaciens SB5	0.25 ± 0.03	0.05 ± 0.004	5.5 ± 0.28	70 ± 0.3	123 ± 0.5	10	This study